1nns

From Proteopedia

(Difference between revisions)

Revision as of 09:23, 16 August 2023

L-asparaginase of E. coli in C2 space group and 1.95 A resolution

Structural highlights

1nns is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.95Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ASPG2_ECOLI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The functional L-asparaginase from Escherichia coli is a homotetramer with a molecular weight of about 142 kDa. The X-ray structure of the enzyme, crystallized in a new form (space group C2) and refined to 1.95 A resolution, is compared with that of the previously determined crystal form (space group P2(1)). The asymmetric unit of the new crystal form contains an L-asparaginase dimer instead of the tetramer found in the previous crystal form. It is found that crystal contacts practically do not affect the conformation of the protein. It is shown that subunit C of the tetrameric form is in a conformation which is systematically different from that of all other subunits in both crystal forms. Major conformational differences are confined to the lid loop (residues 14-27). In addition, the stability of this globular protein is analyzed in terms of the interactions between hydrophobic parts of the subunits.

Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups.,Sanches M, Barbosa JA, de Oliveira RT, Abrahao Neto J, Polikarpov I Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):416-22. Epub 2003, Feb 21. PMID:12595697^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sanches M, Barbosa JA, de Oliveira RT, Abrahao Neto J, Polikarpov I. Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):416-22. Epub 2003, Feb 21. PMID:12595697

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nns"

@@ Line 3: / Line 3: @@
 <StructureSection load='1nns' size='340' side='right'caption='[[1nns]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1nns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1nns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3eca|3eca]], [[1wsa|1wsa]], [[1hfj|1hfj]], [[3pga|3pga]], [[1agx|1agx]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nns OCA], [https://pdbe.org/1nns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nns RCSB], [https://www.ebi.ac.uk/pdbsum/1nns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nns ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/ASPG2_ECOLI ASPG2_ECOLI]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Asparaginase]]
 [[Category: Large Structures]]
-[[Category: Barbosa, J A.R G]]
+[[Category: Barbosa JARG]]
-[[Category: Neto, J A.A]]
+[[Category: Neto JAA]]
-[[Category: Oliveira, R T.de]]
+[[Category: Polikarpov I]]
-[[Category: Polikarpov, I]]
+[[Category: Sanches M]]
-[[Category: Sanches, M]]
+[[Category: De Oliveira RT]]
-[[Category: Amidrohydrolase]]
-[[Category: Crystallographic comparison]]
-[[Category: Hydrolase]]
-[[Category: L-asparaginase]]

1nns

From Proteopedia

Revision as of 09:23, 16 August 2023

L-asparaginase of E. coli in C2 space group and 1.95 A resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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