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| | <StructureSection load='1qgy' size='340' side='right'caption='[[1qgy]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='1qgy' size='340' side='right'caption='[[1qgy]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1qgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QGY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1qgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QGY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PETH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1168 Anabaena PCC7119])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qgy OCA], [https://pdbe.org/1qgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qgy RCSB], [https://www.ebi.ac.uk/pdbsum/1qgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qgy ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qgy OCA], [https://pdbe.org/1qgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qgy RCSB], [https://www.ebi.ac.uk/pdbsum/1qgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qgy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FENR_NOSSO FENR_NOSSO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anabaena pcc7119]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gomez-Moreno, C]] | + | [[Category: Nostoc sp. PCC 7119]] |
| - | [[Category: Hermoso, J A]] | + | [[Category: Gomez-Moreno C]] |
| - | [[Category: Martinez-Julvez, M]] | + | [[Category: Hermoso JA]] |
| - | [[Category: Martinez-Ripoll, M]] | + | [[Category: Martinez-Julvez M]] |
| - | [[Category: Mayoral, T]] | + | [[Category: Martinez-Ripoll M]] |
| - | [[Category: Medina, M]] | + | [[Category: Mayoral T]] |
| - | [[Category: Sanz-Aparicio, J]] | + | [[Category: Medina M]] |
| - | [[Category: Fad]]
| + | [[Category: Sanz-Aparicio J]] |
| - | [[Category: Flavoprotein]]
| + | |
| - | [[Category: Fnr]]
| + | |
| - | [[Category: Nadp]]
| + | |
| - | [[Category: Nadp reductase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
FENR_NOSSO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The three-dimensional structures of K72E, K75R, K75S, K75Q, and K75E Anabaena Ferredoxin-NADP+ reductase (FNR) mutants have been solved, and particular structural details of these mutants have been used to assess the role played by residues 72 and 75 in optimal complex formation and electron transfer (ET) between FNR and its protein redox partners Ferredoxin (Fd) and Flavodoxin (Fld). Additionally, because there is no structural information available on the interaction between FNR and Fld, a model for the FNR:Fld complex has also been produced based on the previously reported crystal structures and on that of the rat Cytochrome P450 reductase (CPR), onto which FNR and Fld have been structurally aligned, and those reported for the Anabaena and maize FNR:Fd complexes. The model suggests putative electrostatic and hydrophobic interactions between residues on the FNR and Fld surfaces at the complex interface and provides an adequate orientation and distance between the FAD and FMN redox centers for efficient ET without the presence of any other molecule as electron carrier. Thus, the models now available for the FNR:Fd and FNR:Fld interactions and the structures presented here for the mutants at K72 and K75 in Anabaena FNR have been evaluated in light of previous biochemical data. These structures confirm the key participation of residue K75 and K72 in complex formation with both Fd and Fld. The drastic effect in FNR activity produced by replacement of K75 by Glu in the K75E FNR variant is explained not only by the observed changes in the charge distribution on the surface of the K75E FNR mutant, but also by the formation of a salt bridge interaction between E75 and K72 that simultaneously "neutralizes" two essential positive charged side chains for Fld/Fd recognition.
Structural analysis of interactions for complex formation between Ferredoxin-NADP+ reductase and its protein partners.,Mayoral T, Martinez-Julvez M, Perez-Dorado I, Sanz-Aparicio J, Gomez-Moreno C, Medina M, Hermoso JA Proteins. 2005 May 15;59(3):592-602. PMID:15789405[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mayoral T, Martinez-Julvez M, Perez-Dorado I, Sanz-Aparicio J, Gomez-Moreno C, Medina M, Hermoso JA. Structural analysis of interactions for complex formation between Ferredoxin-NADP+ reductase and its protein partners. Proteins. 2005 May 15;59(3):592-602. PMID:15789405 doi:http://dx.doi.org/10.1002/prot.20450
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