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| | <StructureSection load='5hyz' size='340' side='right'caption='[[5hyz]], [[Resolution|resolution]] 1.82Å' scene=''> | | <StructureSection load='5hyz' size='340' side='right'caption='[[5hyz]], [[Resolution|resolution]] 1.82Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hyz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HYZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hyz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HYZ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.822Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Os03g0723000, LOC_Os03g51330, OSNPB_030723000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hyz OCA], [http://pdbe.org/5hyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hyz RCSB], [http://www.ebi.ac.uk/pdbsum/5hyz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hyz ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hyz OCA], [https://pdbe.org/5hyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hyz RCSB], [https://www.ebi.ac.uk/pdbsum/5hyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hyz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SCL7_ORYSJ SCL7_ORYSJ] Probable transcription factor involved in plant development (By similarity). Involved in environmental abiotic stress resistance. May increase the expression of stress-responsive genes (By similarity). Binds DNA in vitro (PubMed:27081181).[UniProtKB:A0A024B7I0][UniProtKB:Q9SCR0]<ref>PMID:27081181</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Japanese rice]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Li, S]] | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Sun, L]] | + | [[Category: Li S]] |
| - | [[Category: Wu, Y]] | + | [[Category: Sun L]] |
| - | [[Category: Zhao, Y]] | + | [[Category: Wu Y]] |
| - | [[Category: Rossmann fold helix bundle]]
| + | [[Category: Zhao Y]] |
| - | [[Category: Transcription factor]]
| + | |
| Structural highlights
Function
SCL7_ORYSJ Probable transcription factor involved in plant development (By similarity). Involved in environmental abiotic stress resistance. May increase the expression of stress-responsive genes (By similarity). Binds DNA in vitro (PubMed:27081181).[UniProtKB:A0A024B7I0][UniProtKB:Q9SCR0][1]
Publication Abstract from PubMed
GRAS proteins belong to a plant-specific protein family with many members and play essential roles in plant growth and development, functioning primarily in transcriptional regulation. Proteins in the family are minimally defined as containing the conserved GRAS domain. Here we have determined the structure of the GRAS domain of Os-SCL7 from Oryza sativa to 1.82A. The structure includes cap and core sub-domains, and elucidates the features of the conserved GRAS LRI, VHIID, LRII, PFYRE, and SAW motifs. The structure is a dimer, with a clear groove to accommodate double-stranded DNA. Docking a DNA segment into the groove to generate an Os-SCL7/DNA complex provides insight into the DNA binding mechanism of GRAS proteins. Furthermore, the in vitro DNA binding property of Os-SCL7 and model-defined recognition residues are assessed by EMSA analysis and mutagenesis assays. These studies reveal the structure and preliminary DNA interaction mechanisms of GRAS proteins and open the door to in-depth investigation and understanding of the individual pathways in which they play important roles.
Crystal structure of the GRAS domain of SCARECROW-LIKE 7 in Oryza sativa.,Li S, Zhao Y, Zhao Z, Wu X, Sun L, Liu Q, Wu Y Plant Cell. 2016 Apr 14. pii: tpc.00018.2016. PMID:27081181[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li S, Zhao Y, Zhao Z, Wu X, Sun L, Liu Q, Wu Y. Crystal structure of the GRAS domain of SCARECROW-LIKE 7 in Oryza sativa. Plant Cell. 2016 Apr 14. pii: tpc.00018.2016. PMID:27081181 doi:http://dx.doi.org/10.1105/tpc.16.00018
- ↑ Li S, Zhao Y, Zhao Z, Wu X, Sun L, Liu Q, Wu Y. Crystal structure of the GRAS domain of SCARECROW-LIKE 7 in Oryza sativa. Plant Cell. 2016 Apr 14. pii: tpc.00018.2016. PMID:27081181 doi:http://dx.doi.org/10.1105/tpc.16.00018
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