This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1m55
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1m55.gif|left|200px]] | [[Image:1m55.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1m55", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1m55| PDB=1m55 | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Catalytic domain of the Adeno Associated Virus type 5 Rep protein''' | '''Catalytic domain of the Adeno Associated Virus type 5 Rep protein''' | ||
| Line 29: | Line 26: | ||
[[Category: Kotin, R M.]] | [[Category: Kotin, R M.]] | ||
[[Category: Ronning, D R.]] | [[Category: Ronning, D R.]] | ||
| - | [[Category: | + | [[Category: Adeno-associated virus]] |
| - | [[Category: | + | [[Category: Endonuclease]] |
| - | [[Category: | + | [[Category: Rep]] |
| - | [[Category: | + | [[Category: Rolling circle replication]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:38:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:38, 2 May 2008
Catalytic domain of the Adeno Associated Virus type 5 Rep protein
Overview
Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily.
About this Structure
1M55 is a Single protein structure of sequence from Adeno-associated virus - 5. Full crystallographic information is available from OCA.
Reference
Structural unity among viral origin binding proteins: crystal structure of the nuclease domain of adeno-associated virus Rep., Hickman AB, Ronning DR, Kotin RM, Dyda F, Mol Cell. 2002 Aug;10(2):327-37. PMID:12191478 Page seeded by OCA on Sat May 3 00:38:39 2008
