8pug
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-NTD refinement== | |
| + | <StructureSection load='8pug' size='340' side='right'caption='[[8pug]], [[Resolution|resolution]] 5.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8pug]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_T-cell_leukemia_virus_type_I Human T-cell leukemia virus type I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PUG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pug OCA], [https://pdbe.org/8pug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pug RCSB], [https://www.ebi.ac.uk/pdbsum/8pug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pug ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/GAG_HTL1A GAG_HTL1A] Matrix protein p19 targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p15 is involved in the packaging and encapsidation of two copies of the genome (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here we report the results of cryo-electron tomography (cryo-ET) studies of HTLV-1 virus-like particles (VLPs) assembled in vitro , as well as derived from cells. This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct. | ||
| - | + | Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice.,Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley D, Mansky LM, Dick RA, Schur FK bioRxiv. 2023 Jul 24:2023.07.24.548988. doi: 10.1101/2023.07.24.548988. Preprint. PMID:37546793<ref>PMID:37546793</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Chernikova | + | <div class="pdbe-citations 8pug" style="background-color:#fffaf0;"></div> |
| - | [[Category: Dick | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Human T-cell leukemia virus type I]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Schur | + | [[Category: Chernikova D]] |
| - | [[Category: | + | [[Category: Dick RA]] |
| - | [[Category: | + | [[Category: Mansky LM]] |
| + | [[Category: Obr M]] | ||
| + | [[Category: Percipalle M]] | ||
| + | [[Category: Pinke G]] | ||
| + | [[Category: Porley D]] | ||
| + | [[Category: Schur FKM]] | ||
| + | [[Category: Thader A]] | ||
| + | [[Category: Yang H]] | ||
Revision as of 05:56, 23 August 2023
Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-NTD refinement
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