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| | <StructureSection load='1xb0' size='340' side='right'caption='[[1xb0]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='1xb0' size='340' side='right'caption='[[1xb0]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1xb0]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XB0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1xb0]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XB0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xb1|1xb1]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BIRC8, ILP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xb0 OCA], [https://pdbe.org/1xb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1xb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xb0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xb0 OCA], [http://pdbe.org/1xb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xb0 RCSB], [http://www.ebi.ac.uk/pdbsum/1xb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xb0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | == Disease == | |
| - | [[http://www.uniprot.org/uniprot/DBLOH_HUMAN DBLOH_HUMAN]] Defects in DIABLO are the cause of deafness autosomal dominant type 64 (DFNA64) [MIM:[http://omim.org/entry/614152 614152]]. DFNA64 is a form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.<ref>PMID:21722859</ref> | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BIRC8_HUMAN BIRC8_HUMAN]] Protects against apoptosis mediated by BAX. [[http://www.uniprot.org/uniprot/DBLOH_HUMAN DBLOH_HUMAN]] Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.<ref>PMID:10929711</ref> <ref>PMID:14523016</ref> <ref>PMID:15200957</ref> | + | [https://www.uniprot.org/uniprot/BIRC8_HUMAN BIRC8_HUMAN] Protects against apoptosis mediated by BAX. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 1xb0" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1xb0" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Baculoviral IAP repeat-containing protein 3D structures|Baculoviral IAP repeat-containing protein 3D structures]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Eckelman, B P]] | + | [[Category: Eckelman BP]] |
| - | [[Category: Nunes, V A]] | + | [[Category: Nunes VA]] |
| - | [[Category: Renatus, M]] | + | [[Category: Renatus M]] |
| - | [[Category: Salvesen, G S]] | + | [[Category: Salvesen GS]] |
| - | [[Category: Sampaio, C A.M]] | + | [[Category: Sampaio CAM]] |
| - | [[Category: Shin, H]] | + | [[Category: Shin H]] |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Caspase inhibition]]
| + | |
| - | [[Category: Diablo]]
| + | |
| - | [[Category: Smac]]
| + | |
| - | [[Category: Xiap]]
| + | |
| Structural highlights
Function
BIRC8_HUMAN Protects against apoptosis mediated by BAX.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Several IAP (inhibitor of apoptosis) proteins regulate cell fate decisions, and the X-linked IAP (XIAP) does so in part by inhibiting caspases, proteases that execute the apoptotic pathway. A tissue-specific homologue of XIAP, known as ILP2 (IAP-like protein 2), has previously been implicated in the control of apoptosis in the testis by direct inhibition of caspase 9. In examining this protein we found that the putative caspase 9 interaction domain is a surprisingly weak inhibitor and is also conformationally unstable. Comparison with the equivalent domain in XIAP demonstrated that the instability is due to the lack of a linker segment N-terminal to the inhibitory BIR (baculovirus IAP repeat) domain. Fusion of a 9-residue linker from XIAP to the N-terminus of ILP2 restored tight caspase 9 inhibition, dramatically increased conformational stability and allowed crystallization of the ILP2 BIR domain in a form strikingly similar to the XIAP third BIR domain. We conclude that ILP2 is an unstable protein, and cannot inhibit caspase 9 in a physiological way on its own. We speculate that ILP2 requires assistance from unidentified cellular factors to be an effective inhibitor of apoptosis in vivo.
The BIR domain of IAP-like protein 2 is conformationally unstable: implications for caspase inhibition.,Shin H, Renatus M, Eckelman BP, Nunes VA, Sampaio CA, Salvesen GS Biochem J. 2005 Jan 1;385(Pt 1):1-10. PMID:15485395[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shin H, Renatus M, Eckelman BP, Nunes VA, Sampaio CA, Salvesen GS. The BIR domain of IAP-like protein 2 is conformationally unstable: implications for caspase inhibition. Biochem J. 2005 Jan 1;385(Pt 1):1-10. PMID:15485395 doi:10.1042/BJ20041107
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