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1mc1
From Proteopedia
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[[Image:1mc1.gif|left|200px]] | [[Image:1mc1.gif|left|200px]] | ||
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'''BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI''' | '''BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI''' | ||
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[[Category: Rosenzweig, A C.]] | [[Category: Rosenzweig, A C.]] | ||
[[Category: Townsend, C A.]] | [[Category: Townsend, C A.]] | ||
| - | [[Category: | + | [[Category: Asparagine synthetase]] |
| - | [[Category: | + | [[Category: Beta-lactam synthetase]] |
| - | [[Category: | + | [[Category: Carboxyethyl arginine]] |
| - | [[Category: | + | [[Category: Clavulanic acid]] |
| - | [[Category: | + | [[Category: Deoxyguanidinoproclavaminic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:52:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:52, 2 May 2008
BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI
Overview
The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.
About this Structure
1MC1 is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.
Reference
The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:12409610 Page seeded by OCA on Sat May 3 00:52:35 2008
