5i49

Structural highlights

Function

TUT1_TRYBB Terminal uridylyltransferase which is involved in the post-transcriptional editing of mitochondrial RNA, a process involving the addition and deletion of uridine (U) nucleotides in the pre-RNA (PubMed:11893335, PubMed:20086102, PubMed:26833087, PubMed:27744351). Specifically, catalyzes the addition of Us to the 3'-hydroxyl group of guided RNA (gRNA), ribosomal RNA (rRNA) and some mRNAs (PubMed:11893335, PubMed:20086102, PubMed:26833087, PubMed:27744351). As part of the mitochondrial 3' processome (MPsome), catalyzes the primary 3' uridylation of gRNA precursors to facilitate their recognition and to induce their processive 3'-5' degradation by DSS1, and the secondary 3' uridylation of mature gRNAs (PubMed:26833087). Involved in the 3' uridylylation of the long A/U tail of some edited and never-edited mRNAs (PubMed:20086102). Promotes 3' uridylylation-mediated decay of some never-edited mRNAs (PubMed:20086102). Does not mediate RNA-independent UTP polymerization (PubMed:27744351).^{[1] [2] [3] [4]}

See Also

• RNA uridylyltransferase

References

1. ↑ Aphasizhev R, Sbicego S, Peris M, Jang SH, Aphasizheva I, Simpson AM, Rivlin A, Simpson L. Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing. Cell. 2002 Mar 8;108(5):637-48. PMID:11893335
2. ↑ Aphasizheva I, Aphasizhev R. RET1-catalyzed uridylylation shapes the mitochondrial transcriptome in Trypanosoma brucei. Mol Cell Biol. 2010 Mar;30(6):1555-67. PMID:20086102 doi:10.1128/MCB.01281-09
3. ↑ Suematsu T, Zhang L, Aphasizheva I, Monti S, Huang L, Wang Q, Costello CE, Aphasizhev R. Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial 3' Processome to Generate Guide RNAs. Mol Cell. 2016 Feb 4;61(3):364-378. PMID:26833087 doi:10.1016/j.molcel.2016.01.004
4. ↑ Rajappa-Titu L, Suematsu T, Munoz-Tello P, Long M, Demir O, Cheng KJ, Stagno JR, Luecke H, Amaro RE, Aphasizheva I, Aphasizhev R, Thore S. RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting. Nucleic Acids Res. 2016 Oct 15. pii: gkw917. PMID:27744351 doi:http://dx.doi.org/10.1093/nar/gkw917
5. ↑ Rajappa-Titu L, Suematsu T, Munoz-Tello P, Long M, Demir O, Cheng KJ, Stagno JR, Luecke H, Amaro RE, Aphasizheva I, Aphasizhev R, Thore S. RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting. Nucleic Acids Res. 2016 Oct 15. pii: gkw917. PMID:27744351 doi:http://dx.doi.org/10.1093/nar/gkw917