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| | <StructureSection load='5i4t' size='340' side='right'caption='[[5i4t]], [[Resolution|resolution]] 3.27Å' scene=''> | | <StructureSection load='5i4t' size='340' side='right'caption='[[5i4t]], [[Resolution|resolution]] 3.27Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5i4t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5i4t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I4T FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.273Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4t OCA], [http://pdbe.org/5i4t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4t RCSB], [http://www.ebi.ac.uk/pdbsum/5i4t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4t ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4t OCA], [https://pdbe.org/5i4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i4t RCSB], [https://www.ebi.ac.uk/pdbsum/5i4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4t ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q72497_9HIV1 Q72497_9HIV1] Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Human immunodeficiency virus 1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ganser-Pornillos, B K]] | + | [[Category: Ganser-Pornillos BK]] |
| - | [[Category: Pornillos, O]] | + | [[Category: Pornillos O]] |
| - | [[Category: Wagner, J M]] | + | [[Category: Wagner JM]] |
| - | [[Category: Zadrozny, K]] | + | [[Category: Zadrozny K]] |
| - | [[Category: Capsid]]
| + | |
| - | [[Category: Immature hexagonal lattice]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
Q72497_9HIV1 Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858]
Publication Abstract from PubMed
Virus assembly and maturation proceed through the programmed operation of molecular switches, which trigger both local and global structural rearrangements to produce infectious particles. HIV-1 contains an assembly and maturation switch that spans the C-terminal domain (CTD) of the capsid (CA) region and the first spacer peptide (SP1) of the precursor structural protein, Gag. The crystal structure of the CTD-SP1 Gag fragment is a goblet-shaped hexamer in which the cup comprises the CTD and an ensuing type II beta-turn, and the stem comprises a 6-helix bundle. The beta-turn is critical for immature virus assembly and the 6-helix bundle regulates proteolysis during maturation. This bipartite character explains why the SP1 spacer is a critical element of HIV-1 Gag but is not a universal property of retroviruses. Our results also indicate that HIV-1 maturation inhibitors suppress unfolding of the CA-SP1 junction and thereby delay access of the viral protease to its substrate.
Crystal structure of an HIV assembly and maturation switch.,Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O Elife. 2016 Jul 14;5. pii: e17063. doi: 10.7554/eLife.17063. PMID:27416583[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O. Crystal structure of an HIV assembly and maturation switch. Elife. 2016 Jul 14;5. pii: e17063. doi: 10.7554/eLife.17063. PMID:27416583 doi:http://dx.doi.org/10.7554/eLife.17063
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