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| | <SX load='5i68' size='340' side='right' viewer='molstar' caption='[[5i68]], [[Resolution|resolution]] 3.37Å' scene=''> | | <SX load='5i68' size='340' side='right' viewer='molstar' caption='[[5i68]], [[Resolution|resolution]] 3.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5i68]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I68 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I68 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5i68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I68 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.37Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_oxidase Alcohol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.13 1.1.3.13] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i68 OCA], [http://pdbe.org/5i68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i68 RCSB], [http://www.ebi.ac.uk/pdbsum/5i68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i68 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i68 OCA], [https://pdbe.org/5i68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i68 RCSB], [https://www.ebi.ac.uk/pdbsum/5i68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i68 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ALOX1_KOMPC ALOX1_KOMPC]] Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.<ref>PMID:2657390</ref> <ref>PMID:9396748</ref> | + | [https://www.uniprot.org/uniprot/ALOX1_KOMPG ALOX1_KOMPG] Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.<ref>PMID:9396748</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Alcohol oxidase]] | |
| | [[Category: Komagataella pastoris]] | | [[Category: Komagataella pastoris]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mills, D J]] | + | [[Category: Mills DJ]] |
| - | [[Category: Parcej, D N]] | + | [[Category: Parcej DN]] |
| - | [[Category: Vonck, J]] | + | [[Category: Vonck J]] |
| - | [[Category: Alcohol oxidase peroxisome]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
ALOX1_KOMPG Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.[1]
Publication Abstract from PubMed
The first step in methanol metabolism in methylotrophic yeasts, the oxidation of methanol and higher alcohols with molecular oxygen to formaldehyde and hydrogen peroxide, is catalysed by alcohol oxidase (AOX), a 600-kDa homo-octamer containing eight FAD cofactors. When these yeasts are grown with methanol as the carbon source, AOX forms large crystalline arrays in peroxisomes. We determined the structure of AOX by cryo-electron microscopy at a resolution of 3.4 A. All residues of the 662-amino acid polypeptide as well as the FAD are well resolved. AOX shows high structural homology to other members of the GMC family of oxidoreductases, which share a conserved FAD binding domain, but have different substrate specificities. The preference of AOX for small alcohols is explained by the presence of conserved bulky aromatic residues near the active site. Compared to the other GMC enzymes, AOX contains a large number of amino acid inserts, the longest being 75 residues. These segments are found at the periphery of the monomer and make extensive inter-subunit contacts which are responsible for the very stable octamer. A short surface helix forms contacts between two octamers, explaining the tendency of AOX to form crystals in the peroxisomes.
Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy.,Vonck J, Parcej DN, Mills DJ PLoS One. 2016 Jul 26;11(7):e0159476. doi: 10.1371/journal.pone.0159476., eCollection 2016. PMID:27458710[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Waterham HR, Russell KA, Vries Y, Cregg JM. Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris. J Cell Biol. 1997 Dec 15;139(6):1419-31. PMID:9396748
- ↑ Vonck J, Parcej DN, Mills DJ. Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy. PLoS One. 2016 Jul 26;11(7):e0159476. doi: 10.1371/journal.pone.0159476., eCollection 2016. PMID:27458710 doi:http://dx.doi.org/10.1371/journal.pone.0159476
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