1mgx
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(New page: 200px<br /> <applet load="1mgx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mgx" /> '''COAGULATION FACTOR, MG(II), NMR, 7 STRUCTUR...)
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Revision as of 16:04, 12 November 2007
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COAGULATION FACTOR, MG(II), NMR, 7 STRUCTURES (BACKBONE ATOMS ONLY)
Contents |
Overview
The blood coagulation and regulatory proteins that contain, gamma-carboxyglutamic acid are a part of a unique class of membrane, binding proteins that require calcium for their interaction with cell, membranes. Following protein biosynthesis, glutamic acids on these, proteins are converted to gamma-carboxyglutamic acid (Gla) in a reaction, that requires vitamin K as a cofactor. The vitamin K-dependent proteins, undergo a conformational transition upon metal ion binding, but only, calcium ions mediate protein-phospholipid interaction. To identify the, site on Factor IX that is required for phospholipid binding, we have, determined the three-dimensional structure of the Factor IX Gla domain, bound to magnesium ions by NMR spectroscopy. By comparison of this, structure to that of the Gla domain bound to calcium ions, we localize the, membrane binding site to a highly ordered structure including residues, 1-11 of the Gla domain. In the presence of Ca2+, Factor IX Gla domain, peptides that contain the photoactivatable amino acid, p-benzoyl-L-phenylalanine at positions 6 or 9 cross-link to phospholipid, following irradiation, while peptides lacking this amino acid analog or, with this analog at position 46 did not cross-link. These results indicate, that the NH2 terminus of the Gla domain, specifically including leucine 6, and phenylalanine 9 in the hydrophobic patch, is the contact surface on, Factor IX that interacts with the phospholipid bilayer.
Disease
Known diseases associated with this structure: Hemophilia B OMIM:[306900], Warfarin sensitivity OMIM:[306900]
About this Structure
1MGX is a Single protein structure of sequence from Homo sapiens. Active as Coagulation factor IXa, with EC number 3.4.21.22 Full crystallographic information is available from OCA.
Reference
Identification of the phospholipid binding site in the vitamin K-dependent blood coagulation protein factor IX., Freedman SJ, Blostein MD, Baleja JD, Jacobs M, Furie BC, Furie B, J Biol Chem. 1996 Jul 5;271(27):16227-36. PMID:8663165
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