1mdv
From Proteopedia
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'''KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES''' | '''KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES''' | ||
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[[Category: Makarov, A.]] | [[Category: Makarov, A.]] | ||
[[Category: Protasevich, I.]] | [[Category: Protasevich, I.]] | ||
| - | [[Category: | + | [[Category: Desulfovibrio vulgaris hildenborough]] |
| - | [[Category: | + | [[Category: Electron transport]] |
| - | [[Category: | + | [[Category: Mutant cytochrome c3]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:55:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:55, 2 May 2008
KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES
Overview
Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost parallel to the ring plane of heme 1. We replaced this residue by a nonaromatic hydrophobe residue, leucine, and analyzed the effects in terms of functional, structural, and physicochemical properties. While the F20L replacement did not have any strong effects on the heme region stability, a decrease of the thermostability of the whole molecule was observed. In the same way, the four macroscopic redox potentials were affected by the mutation as well as the flexibility of the surface loop around heme 4. The F20L replacement itself and/or this structural modification might be responsible for the loss of the intermolecular cooperativity between F20L cytochrome c3 molecules.
About this Structure
1MDV is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.
Reference
Key role of phenylalanine 20 in cytochrome c3: structure, stability, and function studies., Dolla A, Arnoux P, Protasevich I, Lobachov V, Brugna M, Giudici-Orticoni MT, Haser R, Czjzek M, Makarov A, Bruschi M, Biochemistry. 1999 Jan 5;38(1):33-41. PMID:9890880 Page seeded by OCA on Sat May 3 00:55:29 2008
