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1mh9
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(New page: 200px<br /> <applet load="1mh9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mh9, resolution 1.80Å" /> '''Crystal Structure A...)
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Revision as of 16:04, 12 November 2007
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Crystal Structure Analysis of deoxyribonucleotidase
Overview
5' nucleotidases are ubiquitous enzymes that dephosphorylate nucleoside, monophosphates and participate in the regulation of nucleotide pools. The, mitochondrial 5'-(3') deoxyribonucleotidase (dNT-2) specifically, dephosphorylates dUMP and dTMP, thereby protecting mitochondrial DNA, replication from excess dTTP. We have solved the structure of dNT-2, the, first of a mammalian 5' nucleotidase. The structure reveals a relationship, to the HAD family, members of which use an aspartyl nucleophile as their, common catalytic strategy, with a phosphoserine phosphatase as the most, similar neighbor. A structure-based sequence alignment of dNT-2 with other, 5' nucleotidases also suggests a common origin for these enzymes. Here we, study the structures of dNT-2 in complex with bound phosphate and, beryllium trifluoride plus thymidine as model for a phosphoenzyme-product, complex. Based on these structures, determinants for substrate specificity, recognition and the catalytic action of dNT-2 are outlined.
About this Structure
1MH9 is a Single protein structure of sequence from Homo sapiens with PO4 and MG as ligands. Active as 5'-nucleotidase, with EC number 3.1.3.5 Full crystallographic information is available from OCA.
Reference
Crystal structure of a human mitochondrial deoxyribonucleotidase., Rinaldo-Matthis A, Rampazzo C, Reichard P, Bianchi V, Nordlund P, Nat Struct Biol. 2002 Oct;9(10):779-87. PMID:12352955
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