2gim

From Proteopedia

(Difference between revisions)

Current revision

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

Structural highlights

2gim is a 2 chain structure with sequence from Trichormus variabilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLAS_ANAVA Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

Structure of plastocyanin from the cyanobacterium Anabaena variabilis.,Schmidt L, Christensen HE, Harris P Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schmidt L, Christensen HE, Harris P. Structure of plastocyanin from the cyanobacterium Anabaena variabilis. Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103 doi:10.1107/S0907444906023638

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gim"

Categories: Large Structures | Trichormus variabilis | Christensen HEM | Harris P | Schmidt L

@@ Line 3: / Line 3: @@
 <StructureSection load='2gim' size='340' side='right'caption='[[2gim]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anava Anava]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichormus_variabilis Trichormus variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 ANAVA])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gim OCA], [https://pdbe.org/2gim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gim RCSB], [https://www.ebi.ac.uk/pdbsum/2gim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gim ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PLAS_ANAVA PLAS_ANAVA]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
+[https://www.uniprot.org/uniprot/PLAS_ANAVA PLAS_ANAVA] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 2gim" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Anava]]
 [[Category: Large Structures]]
-[[Category: Christensen, H E.M]]
+[[Category: Trichormus variabilis]]
-[[Category: Harris, P]]
+[[Category: Christensen HEM]]
-[[Category: Schmidt, L]]
+[[Category: Harris P]]
-[[Category: Beta sheet]]
+[[Category: Schmidt L]]
-[[Category: Cu]]
-[[Category: Electron transport]]
-[[Category: Helix]]

2gim

From Proteopedia

Current revision

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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