2gsq

From Proteopedia

(Difference between revisions)

Current revision

GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE

Structural highlights

2gsq is a 1 chain structure with sequence from Nototodarus sloanii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GST_NOTSL High activity toward 1-chloro-2,4-dinitrobenzene. Not very efficient at catalyzing the addition of GSH to enones and epoxides.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.

Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.,Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ji X, von Rosenvinge EC, Johnson WW, Tomarev SI, Piatigorsky J, Armstrong RN, Gilliland GL. Three-dimensional structure, catalytic properties, and evolution of a sigma class glutathione transferase from squid, a progenitor of the lens S-crystallins of cephalopods. Biochemistry. 1995 Apr 25;34(16):5317-28. PMID:7727393
↑ Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL. Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gsq"

Categories: Large Structures | Nototodarus sloanii | Armstrong RN | Gilliland GL | Ji X

@@ Line 3: / Line 3: @@
 <StructureSection load='2gsq' size='340' side='right'caption='[[2gsq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gsq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gsq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nototodarus_sloanii Nototodarus sloanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GBI:S-(3-IODOBENZYL)GLUTATHIONE'>GBI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GBI:S-(3-IODOBENZYL)GLUTATHIONE'>GBI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsq OCA], [https://pdbe.org/2gsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gsq RCSB], [https://www.ebi.ac.uk/pdbsum/2gsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gsq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/GST_NOTSL GST_NOTSL]] High activity toward 1-chloro-2,4-dinitrobenzene. Not very efficient at catalyzing the addition of GSH to enones and epoxides.<ref>PMID:7727393</ref>
+[https://www.uniprot.org/uniprot/GST_NOTSL GST_NOTSL] High activity toward 1-chloro-2,4-dinitrobenzene. Not very efficient at catalyzing the addition of GSH to enones and epoxides.<ref>PMID:7727393</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Glutathione transferase]]
 [[Category: Large Structures]]
-[[Category: Armstrong, R N]]
+[[Category: Nototodarus sloanii]]
-[[Category: Gilliland, G L]]
+[[Category: Armstrong RN]]
-[[Category: Ji, X]]
+[[Category: Gilliland GL]]
-[[Category: Sigma class]]
+[[Category: Ji X]]
-[[Category: Squid digestive gland]]
-[[Category: Transferase]]

2gsq

From Proteopedia

Current revision

GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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