1miu

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Revision as of 16:05, 12 November 2007

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spinqualitylabelsall models 1miu, resolution 3.1Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of a BRCA2-DSS1 complex

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Mutations in the BRCA2 (breast cancer susceptibility gene 2) tumor, suppressor lead to chromosomal instability due to defects in the repair of, double-strand DNA breaks (DSBs) by homologous recombination, but BRCA2's, role in this process has been unclear. Here, we present the 3.1 angstrom, crystal structure of a approximately 90-kilodalton BRCA2 domain bound to, DSS1, which reveals three oligonucleotide-binding (OB) folds and a, helix-turn-helix (HTH) motif. We also (i) demonstrate that this BRCA2, domain binds single-stranded DNA, (ii) present its 3.5 angstrom structure, bound to oligo(dT)9, (iii) provide data that implicate the HTH motif in, dsDNA binding, and (iv) show that BRCA2 stimulates RAD51-mediated, recombination in vitro. These findings establish that BRCA2 functions, directly in homologous recombination and provide a structural and, biochemical basis for understanding the loss of recombination-mediated DSB, repair in BRCA2-associated cancers.

Disease

Known disease associated with this structure: Split hand/foot malformation, type 1 OMIM:[183600]

About this Structure

1MIU is a Protein complex structure of sequences from Homo sapiens and Mus musculus with HG as ligand. Full crystallographic information is available from OCA.

Reference

BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure., Yang H, Jeffrey PD, Miller J, Kinnucan E, Sun Y, Thoma NH, Zheng N, Chen PL, Lee WH, Pavletich NP, Science. 2002 Sep 13;297(5588):1837-48. PMID:12228710

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