We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2hfk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:57, 30 August 2023) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='2hfk' size='340' side='right'caption='[[2hfk]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
<StructureSection load='2hfk' size='340' side='right'caption='[[2hfk]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2hfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1526 As 4.1526]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HFK FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2hfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HFK FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E4H:(3R,4S,5S,7R,9E,11R,12R)-12-ETHYL-4-HYDROXY-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE'>E4H</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mna|1mna]], [[2hfj|2hfj]]</div></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E4H:(3R,4S,5S,7R,9E,11R,12R)-12-ETHYL-4-HYDROXY-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE'>E4H</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pikAIV ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54571 AS 4.1526])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hfk OCA], [https://pdbe.org/2hfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hfk RCSB], [https://www.ebi.ac.uk/pdbsum/2hfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hfk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hfk OCA], [https://pdbe.org/2hfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hfk RCSB], [https://www.ebi.ac.uk/pdbsum/2hfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hfk ProSAT]</span></td></tr>
</table>
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PIKA4_STRVZ PIKA4_STRVZ] Involved in the biosynthesis of 12- and 14-membered ring macrolactone antibiotics such as methymycin and neomethymycin, and pikromycin and narbomycin, respectively. Component of the pikromycin PKS which catalyzes the biosynthesis of both precursors 10-deoxymethynolide (12-membered ring macrolactone) and narbonolide (14-membered ring macrolactone). Chain elongation through PikAI, PikAII and PikAIII followed by thioesterase catalyzed termination results in the production of 10-deoxymethynolide, while continued elongation through PikAIV, followed by thioesterase (TE) catalyzed cyclization results in the biosynthesis of the narbonolide. The thioesterase can use a series of diketide-N-acetylcysteamine (SNAC) thioesters, but has a strong preference for the 2-methyl-3-ketopentanoyl-SNAC over the stereoisomers of 2-methyl-3-hydroxyacyl-SNAC (PubMed:12379101, PubMed:12733905).<ref>PMID:10421766</ref> <ref>PMID:10676969</ref> <ref>PMID:12379101</ref> <ref>PMID:12733905</ref> <ref>PMID:16969372</ref> <ref>PMID:17719493</ref> <ref>PMID:19027305</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 32: Line 33:
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: As 4 1526]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Akey, D L]]
+
[[Category: Streptomyces venezuelae]]
-
[[Category: Fecik, R A]]
+
[[Category: Akey DL]]
-
[[Category: Giraldes, J W]]
+
[[Category: Fecik RA]]
-
[[Category: Kittendorf, J D]]
+
[[Category: Giraldes JW]]
-
[[Category: Sherman, D H]]
+
[[Category: Kittendorf JD]]
-
[[Category: Smith, J L]]
+
[[Category: Sherman DH]]
-
[[Category: Alpha/beta hydrolase]]
+
[[Category: Smith JL]]
-
[[Category: Hydrolase]]
+
-
[[Category: Thioesterase]]
+

Current revision

Pikromycin thioesterase in complex with product 10-deoxymethynolide

PDB ID 2hfk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hfk"
Personal tools