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| | <StructureSection load='2nn2' size='340' side='right'caption='[[2nn2]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='2nn2' size='340' side='right'caption='[[2nn2]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2nn2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NN2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2nn2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NN2 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ZBTB7A, FBI1, LRF, ZBTB7 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nn2 OCA], [https://pdbe.org/2nn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2nn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nn2 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nn2 OCA], [https://pdbe.org/2nn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2nn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nn2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ZBT7A_HUMAN ZBT7A_HUMAN]] Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).
| + | [https://www.uniprot.org/uniprot/ZBT7A_HUMAN ZBT7A_HUMAN] Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, L]] | + | [[Category: Chen L]] |
| - | [[Category: Prive, G G]] | + | [[Category: Prive GG]] |
| - | [[Category: Stogios, P J]] | + | [[Category: Stogios PJ]] |
| - | [[Category: Btb domain]]
| + | |
| - | [[Category: Candidate oncoprotein]]
| + | |
| - | [[Category: Poz domain]]
| + | |
| - | [[Category: Protein-protein interaction domain]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription repressor]]
| + | |
| - | [[Category: Zinc-finger protein]]
| + | |
| Structural highlights
Function
ZBT7A_HUMAN Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
BTB-zinc finger (BTB-ZF) proteins are transcription regulators with roles in development, differentiation, and oncogenesis. In these proteins, the BTB domain (also known as the POZ domain) is a protein-protein interaction motif that contains a dimerization interface, a possible oligomerization surface, and surfaces for interactions with other factors, including nuclear co-repressors and histone deacetylases. The BTB-ZF protein LRF (also known as ZBTB7, FBI-1, OCZF, and Pokemon) is a master regulator of oncogenesis, and represses the transcription of a variety of important genes, including the ARF, c-fos, and c-myc oncogenes and extracellular matrix genes. We determined the crystal structure of the BTB domain from human LRF to 2.1 A and observed the canonical BTB homodimer fold. However, novel features are apparent on the surface of the homodimer, including differences in the lateral groove and charged pocket regions. The residues that line the lateral groove have little similarity with the equivalent residues from the BCL6 BTB domain, and we show that the 17-residue BCL6 Binding Domain (BBD) from the SMRT co-repressor does not bind to the LRF BTB domain.
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.,Stogios PJ, Chen L, Prive GG Protein Sci. 2007 Feb;16(2):336-42. Epub 2006 Dec 22. PMID:17189472[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stogios PJ, Chen L, Prive GG. Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator. Protein Sci. 2007 Feb;16(2):336-42. Epub 2006 Dec 22. PMID:17189472 doi:10.1110/ps.062660907
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