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| | <StructureSection load='2ov3' size='340' side='right'caption='[[2ov3]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='2ov3' size='340' side='right'caption='[[2ov3]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ov3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanobacterium_synechocystis Cyanobacterium synechocystis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OV3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ov3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OV3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ov1|2ov1]], [[1pq4|1pq4]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zntC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1143 Cyanobacterium synechocystis])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ov3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ov3 OCA], [https://pdbe.org/2ov3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ov3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ov3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ov3 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ov3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ov3 OCA], [https://pdbe.org/2ov3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ov3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ov3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ov3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P73085_SYNY3 P73085_SYNY3] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cyanobacterium synechocystis]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Smith, T J]] | + | [[Category: Synechocystis sp]] |
| - | [[Category: Wei, B]] | + | [[Category: Smith TJ]] |
| - | [[Category: Abc transporter]] | + | [[Category: Wei B]] |
| - | [[Category: Solute binding domain]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Zinc transport]]
| + | |
| Structural highlights
Function
P73085_SYNY3
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A number of bacterial metal transporters belong to the ABC transporter family. To better understand the structural determinants of metal selectivity of one such transporter, we previously determined the structure of the periplasmic domain of a zinc transporter, ZnuA, from Synechocystis 6803 and found that ZnuA binds zinc via three histidines. Unique to these ABC zinc transporters, ZnuA has a highly charged and mobile loop that protrudes from the protein in the vicinity of the metal binding site that we had suggested might facilitate zinc acquisition. To further examine the function of this loop, the structure and zinc binding properties of two ZnuA variants were determined. When the loop is entirely deleted, zinc still binds to the three histidines. However, unlike what was suggested from the structure of a similar solute binding protein, TroA, release of zinc occurs concomitantly with large conformational changes in two of the three chelating histidines. These structural results combined with isothermal titration calorimetry data demonstrate that there are at least two classes of zinc binding sites: the high-affinity site in the cleft between the two domains and at least one additional site on the flexible loop. This loop has approximately 100-fold weaker affinity for zinc than the high-affinity zinc binding site, and its deletion does not affect the high-affinity site. From these results, we suggest that this region might be a sensor for high periplasmic levels of zinc.
Possible regulatory role for the histidine-rich loop in the zinc transport protein, ZnuA.,Wei B, Randich AM, Bhattacharyya-Pakrasi M, Pakrasi HB, Smith TJ Biochemistry. 2007 Jul 31;46(30):8734-43. Epub 2007 Jul 6. PMID:17616151[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wei B, Randich AM, Bhattacharyya-Pakrasi M, Pakrasi HB, Smith TJ. Possible regulatory role for the histidine-rich loop in the zinc transport protein, ZnuA. Biochemistry. 2007 Jul 31;46(30):8734-43. Epub 2007 Jul 6. PMID:17616151 doi:10.1021/bi700763w
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