This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1mis
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1mis.jpg|left|200px]] | [[Image:1mis.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1mis", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1mis| PDB=1mis | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE''' | '''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIS OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703939 8703939] | Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703939 8703939] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Turner, D H.]] | [[Category: Turner, D H.]] | ||
[[Category: Wu, M.]] | [[Category: Wu, M.]] | ||
| - | [[Category: | + | [[Category: Ribonucleic acid]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | [[Category: | + | [[Category: Tandem g:a mismatch]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:07:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:07, 2 May 2008
STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939 Page seeded by OCA on Sat May 3 01:07:44 2008
