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| | <StructureSection load='2q73' size='340' side='right'caption='[[2q73]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='2q73' size='340' side='right'caption='[[2q73]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2q73]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_sp._dat722 Vibrio sp. dat722]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q73 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2q73]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_sp._DAT722 Vibrio sp. DAT722]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q73 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2q5z|2q5z]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">imazG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=344879 Vibrio sp. DAT722])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nucleoside-triphosphate_diphosphatase Nucleoside-triphosphate diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.19 3.6.1.19] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q73 OCA], [https://pdbe.org/2q73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q73 RCSB], [https://www.ebi.ac.uk/pdbsum/2q73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q73 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q73 OCA], [https://pdbe.org/2q73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q73 RCSB], [https://www.ebi.ac.uk/pdbsum/2q73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q73 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2F9Z1_9VIBR Q2F9Z1_9VIBR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nucleoside-triphosphate diphosphatase]]
| + | [[Category: Vibrio sp. DAT722]] |
| - | [[Category: Vibrio sp. dat722]] | + | [[Category: Boucher Y]] |
| - | [[Category: Boucher, Y]] | + | [[Category: Curmi PMG]] |
| - | [[Category: Curmi, P M.G]] | + | [[Category: Guilfoyle AP]] |
| - | [[Category: Guilfoyle, A P]] | + | [[Category: Harrop SJ]] |
| - | [[Category: Harrop, S J]] | + | [[Category: Mabbutt BC]] |
| - | [[Category: Mabbutt, B C]] | + | [[Category: Robinson A]] |
| - | [[Category: Robinson, A]] | + | [[Category: Stokes HW]] |
| - | [[Category: Stokes, H W]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Mazg]]
| + | |
| - | [[Category: Ntp-ppase]]
| + | |
| - | [[Category: Vibrio]]
| + | |
| Structural highlights
Function
Q2F9Z1_9VIBR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mobile gene cassettes collectively contain a highly diverse pool of novel genes that encode many novel adaptive functions. In the non-clinical context, the function of almost all of the encoded proteins remains unknown despite the enormous size of this mobile gene pool. We have been characterizing cassette arrays by taking advantage of the fact that they cluster at discrete sites in chromosomes; even large arrays are thus recoverable in a relatively small number of clones in genomic libraries. In one assembled array of 116 cassettes from the marine bacterium Vibrio sp. DAT722, a putative MazG protein is encoded within the 21st cassette. Because MazG proteins are implicated in a number of cellular processes, including house-cleaning and stress survival, the presence of such a protein in a mobile cassette was noteworthy. Here we solve the crystal structure of this alpha-helical protein, and define both open and closed states of a new variant of the MazG family. Functional assays confirm that the protein is a dNTP pyrophosphohydrolase, with marked preferences for dCTP and dATP. We hypothesize that iMazG acts as a house-cleaning enzyme, preventing the incorporation of damaging non-canonical nucleotides into host-cell DNA.
A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype.,Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC Mol Microbiol. 2007 Nov;66(3):610-21. Epub 2007 Sep 24. PMID:17892463[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC. A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype. Mol Microbiol. 2007 Nov;66(3):610-21. Epub 2007 Sep 24. PMID:17892463 doi:10.1111/j.1365-2958.2007.05932.x
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