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| | <StructureSection load='3drz' size='340' side='right'caption='[[3drz]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='3drz' size='340' side='right'caption='[[3drz]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3drz]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DRZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3drz]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DRZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3drx|3drx]], [[3dry|3dry]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCTD5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3drz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3drz OCA], [https://pdbe.org/3drz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3drz RCSB], [https://www.ebi.ac.uk/pdbsum/3drz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3drz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3drz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3drz OCA], [https://pdbe.org/3drz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3drz RCSB], [https://www.ebi.ac.uk/pdbsum/3drz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3drz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/KCTD5_HUMAN KCTD5_HUMAN]] Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.<ref>PMID:18573101</ref>
| + | [https://www.uniprot.org/uniprot/KCTD5_HUMAN KCTD5_HUMAN] Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.<ref>PMID:18573101</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dementieva, I]] | + | [[Category: Dementieva I]] |
| - | [[Category: Goldstein, S A.N]] | + | [[Category: Goldstein SAN]] |
| - | [[Category: Tereshko, V]] | + | [[Category: Tereshko V]] |
| - | [[Category: Btb/poz]]
| + | |
| - | [[Category: Kctd5]]
| + | |
| - | [[Category: Pentamer]]
| + | |
| - | [[Category: Potassium channel domain t1]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
KCTD5_HUMAN Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We report the X-ray crystal structure of human potassium channel tetramerization domain-containing protein 5 (KCTD5), the first member of the family to be so characterized. Four findings were unexpected. First, the structure reveals assemblies of five subunits while tetramers were anticipated; pentameric stoichiometry is observed also in solution by scanning transmission electron microscopy mass analysis and analytical ultracentrifugation. Second, the same BTB (bric-a-brac, tramtrack, broad complex) domain surface mediates the assembly of five KCTD5 and four voltage-gated K(+) (Kv) channel subunits; four amino acid differences appear crucial. Third, KCTD5 complexes have well-defined N- and C-terminal modules separated by a flexible linker that swivels by approximately 30 degrees; the C-module shows a new fold and is required to bind Golgi reassembly stacking protein 55 with approximately 1 microM affinity, as judged by surface plasmon resonance and ultracentrifugation. Fourth, despite the homology reflected in its name, KCTD5 does not impact the operation of Kv4.2, Kv3.4, Kv2.1, or Kv1.2 channels.
Pentameric assembly of potassium channel tetramerization domain-containing protein 5.,Dementieva IS, Tereshko V, McCrossan ZA, Solomaha E, Araki D, Xu C, Grigorieff N, Goldstein SA J Mol Biol. 2009 Mar 20;387(1):175-91. Epub 2009 Jan 23. PMID:19361449[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bayon Y, Trinidad AG, de la Puerta ML, Del Carmen Rodriguez M, Bogetz J, Rojas A, De Pereda JM, Rahmouni S, Williams S, Matsuzawa S, Reed JC, Crespo MS, Mustelin T, Alonso A. KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases. FEBS J. 2008 Aug;275(15):3900-10. doi: 10.1111/j.1742-4658.2008.06537.x. Epub, 2008 Jun 28. PMID:18573101 doi:http://dx.doi.org/10.1111/j.1742-4658.2008.06537.x
- ↑ Dementieva IS, Tereshko V, McCrossan ZA, Solomaha E, Araki D, Xu C, Grigorieff N, Goldstein SA. Pentameric assembly of potassium channel tetramerization domain-containing protein 5. J Mol Biol. 2009 Mar 20;387(1):175-91. Epub 2009 Jan 23. PMID:19361449 doi:10.1016/j.jmb.2009.01.030
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