|
|
Line 3: |
Line 3: |
| <StructureSection load='3e0w' size='340' side='right'caption='[[3e0w]], [[Resolution|resolution]] 3.10Å' scene=''> | | <StructureSection load='3e0w' size='340' side='right'caption='[[3e0w]], [[Resolution|resolution]] 3.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[3e0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leime Leime]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E0W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3e0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_mexicana Leishmania mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E0W FirstGlance]. <br> |
- | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3e0v|3e0v]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5665 LEIME])</td></tr>
| + | |
- | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
| + | |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e0w OCA], [https://pdbe.org/3e0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e0w RCSB], [https://www.ebi.ac.uk/pdbsum/3e0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e0w ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e0w OCA], [https://pdbe.org/3e0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e0w RCSB], [https://www.ebi.ac.uk/pdbsum/3e0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e0w ProSAT]</span></td></tr> |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/KPYK_LEIME KPYK_LEIME] |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
Line 36: |
Line 36: |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Leime]] | + | [[Category: Leishmania mexicana]] |
- | [[Category: Pyruvate kinase]]
| + | [[Category: Gillmore LA]] |
- | [[Category: Gillmore, L A]] | + | [[Category: Tulloch LB]] |
- | [[Category: Tulloch, L B]] | + | [[Category: Walkinshaw MD]] |
- | [[Category: Walkinshaw, M D]] | + | |
- | [[Category: Adp]]
| + | |
- | [[Category: Allosteric enzyme]]
| + | |
- | [[Category: Glycolysis]]
| + | |
- | [[Category: Kinase]]
| + | |
- | [[Category: Leishmania]]
| + | |
- | [[Category: Magnesium]]
| + | |
- | [[Category: Metal-binding]]
| + | |
- | [[Category: Mexicana]]
| + | |
- | [[Category: Nad+ nadh]]
| + | |
- | [[Category: Pep]]
| + | |
- | [[Category: Phosphoenolpyruvate]]
| + | |
- | [[Category: Pyruvate]]
| + | |
- | [[Category: Transferase]]
| + | |
- | [[Category: Trypanosomatid]]
| + | |
| Structural highlights
Function
KPYK_LEIME
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We report X-ray structures of pyruvate kinase from Leishmania mexicana (LmPYK) that are trapped in different conformations. These, together with the previously reported structure of LmPYK in its inactive (T-state) conformation, allow comparisons of three different conformers of the same species of pyruvate kinase (PYK). Four new site point mutants showing the effects of side-chain alteration at subunit interfaces are also enzymatically characterised. The LmPYK tetramer crystals grown with ammonium sulphate as precipitant adopt an active-like conformation, with sulphate ions at the active and effector sites. The sulphates occupy positions similar to those of the phosphates of ligands bound to active (R-state) and constitutively active (nonallosteric) PYKs from several species, and provide insight into the structural roles of the phosphates of the substrates and effectors. Crystal soaking in sulphate-free buffers was found to induce major conformational changes in the tetramer. In particular, the unwinding of the Aalpha6' helix and the inward hinge movement of the B domain are coupled with a significant widening (4 A) of the tetramer caused by lateral movement of the C domains. The two new LmPYK structures and the activity studies of site point mutations described in this article are consistent with a developing picture of allosteric activity in which localised changes in protein flexibility govern the distribution of conformer families adopted by the tetramer in its active and inactive states.
Sulphate removal induces a major conformational change in Leishmania mexicana pyruvate kinase in the crystalline state.,Tulloch LB, Morgan HP, Hannaert V, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD J Mol Biol. 2008 Nov 14;383(3):615-26. Epub 2008 Aug 23. PMID:18775437[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tulloch LB, Morgan HP, Hannaert V, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD. Sulphate removal induces a major conformational change in Leishmania mexicana pyruvate kinase in the crystalline state. J Mol Biol. 2008 Nov 14;383(3):615-26. Epub 2008 Aug 23. PMID:18775437 doi:10.1016/j.jmb.2008.08.037
|