1mlx

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[[Image:1mlx.gif|left|200px]]
[[Image:1mlx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1mlx |SIZE=350|CAPTION= <scene name='initialview01'>1mlx</scene>, resolution 1.25&Aring;
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The line below this paragraph, containing "STRUCTURE_1mlx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SMT:2&#39;-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5&#39;-MONOPHOSPHATE'>SMT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|DOMAIN=
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{{STRUCTURE_1mlx| PDB=1mlx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB]</span>
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'''Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex'''
'''Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex'''
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==About this Structure==
==About this Structure==
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1MLX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA].
==Reference==
==Reference==
2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12269806 12269806]
2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12269806 12269806]
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[[Category: Protein complex]]
 
[[Category: Egli, M.]]
[[Category: Egli, M.]]
[[Category: Fraser, A S.]]
[[Category: Fraser, A S.]]
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[[Category: Sioufi, N.]]
[[Category: Sioufi, N.]]
[[Category: Teplova, M.]]
[[Category: Teplova, M.]]
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[[Category: a-form]]
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[[Category: A-form]]
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[[Category: antisense modification]]
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[[Category: Antisense modification]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: high resolution structure]]
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[[Category: High resolution structure]]
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[[Category: hydration]]
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[[Category: Hydration]]
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[[Category: nuclease resistance]]
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[[Category: Nuclease resistance]]
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[[Category: nucleic acid analogue]]
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[[Category: Nucleic acid analogue]]
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[[Category: protein binding affinity]]
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[[Category: Protein binding affinity]]
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[[Category: rna affinity]]
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[[Category: Rna affinity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:23:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:17:50 2008''
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Revision as of 22:23, 2 May 2008

Image:1mlx.gif

Template:STRUCTURE 1mlx

Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex


Overview

A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.

About this Structure

Full crystallographic information is available from OCA.

Reference

2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806 Page seeded by OCA on Sat May 3 01:23:51 2008

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