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| | ==Crystal structure of protease domain of Deg2 linked with the PDZ domain of Deg9== | | ==Crystal structure of protease domain of Deg2 linked with the PDZ domain of Deg9== |
| - | <StructureSection load='5ilb' size='340' side='right' caption='[[5ilb]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='5ilb' size='340' side='right'caption='[[5ilb]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ilb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ILB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ILB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ilb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ILB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ILB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5il9|5il9]], [[5ila|5ila]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.852Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DEGP9, At5g40200, MSN9.10, MSN9.100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ilb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ilb OCA], [https://pdbe.org/5ilb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ilb RCSB], [https://www.ebi.ac.uk/pdbsum/5ilb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ilb ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ilb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ilb OCA], [http://pdbe.org/5ilb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ilb RCSB], [http://www.ebi.ac.uk/pdbsum/5ilb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ilb ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DEGP2_ARATH DEGP2_ARATH]] Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II.<ref>PMID:11179216</ref> | + | [https://www.uniprot.org/uniprot/DEGP2_ARATH DEGP2_ARATH] Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II.<ref>PMID:11179216</ref> [https://www.uniprot.org/uniprot/DEGP9_ARATH DEGP9_ARATH] Probable serine protease. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Li, X Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Liu, L]] | + | [[Category: Li XY]] |
| - | [[Category: Ouyang, M]] | + | [[Category: Liu L]] |
| - | [[Category: Zhang, L X]] | + | [[Category: Ouyang M]] |
| - | [[Category: Zhao, S]] | + | [[Category: Zhang LX]] |
| - | [[Category: Deg2]]
| + | [[Category: Zhao S]] |
| - | [[Category: Deg9]]
| + | |
| - | [[Category: Fusion protein]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
DEGP2_ARATH Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II.[1] DEGP9_ARATH Probable serine protease.
Publication Abstract from PubMed
The high temperature requirement A (HtrA) proteases (also termed Deg proteases) play important roles in diverse organisms by regulating protein quality and quantity. One of the 16 Arabidopsis homologs, Deg9, is located in the nucleus where it modulates cytokinin- and light-mediated signalling via degrading the ARABIDOPSIS RESPONSE REGULATOR 4 (ARR4). To uncover the structural features underlying the proteolytic activity of Deg9, we determined its crystal structure. Unlike the well-established trimeric building block of HtrAs, Deg9 displays a novel octameric structure consisting of two tetrameric rings that have distinct conformations. Based on the structural architecture, we generated several mutant variants of Deg9, determined their structure and tested their proteolytic activity towards ARR4. The results of the structural and biochemical analyses allowed us to propose a model for a novel mechanism of substrate recognition and activity regulation of Deg9. In this model, protease activation of one tetramer is mediated by en-bloc reorientation of the protease domains to open an entrance for the substrate in the opposite (inactive) tetramer. This study provides the structural basis for understanding how the levels of nuclear signal components are regulated by a plant protease.
The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.,Ouyang M, Li X, Zhao S, Pu H, Shen J, Adam Z, Clausen T, Zhang L Nat Plants. 2017 Dec;3(12):973-982. doi: 10.1038/s41477-017-0060-2. Epub 2017 Nov, 27. PMID:29180814[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Haussuhl K, Andersson B, Adamska I. A chloroplast DegP2 protease performs the primary cleavage of the photodamaged D1 protein in plant photosystem II. EMBO J. 2001 Feb 15;20(4):713-22. PMID:11179216 doi:http://dx.doi.org/10.1093/emboj/20.4.713
- ↑ Ouyang M, Li X, Zhao S, Pu H, Shen J, Adam Z, Clausen T, Zhang L. The crystal structure of Deg9 reveals a novel octameric-type HtrA protease. Nat Plants. 2017 Dec;3(12):973-982. doi: 10.1038/s41477-017-0060-2. Epub 2017 Nov, 27. PMID:29180814 doi:http://dx.doi.org/10.1038/s41477-017-0060-2
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