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| | <StructureSection load='3et9' size='340' side='right'caption='[[3et9]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='3et9' size='340' side='right'caption='[[3et9]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3et9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ET9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ET9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3et9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ET9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ET9 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3esu|3esu]], [[3esv|3esv]], [[3etb|3etb]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3et9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3et9 OCA], [https://pdbe.org/3et9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3et9 RCSB], [https://www.ebi.ac.uk/pdbsum/3et9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3et9 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3et9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3et9 OCA], [https://pdbe.org/3et9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3et9 RCSB], [https://www.ebi.ac.uk/pdbsum/3et9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3et9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Barnett, J]] | + | [[Category: Barnett J]] |
| - | [[Category: Georgiou, G]] | + | [[Category: Georgiou G]] |
| - | [[Category: Iverson, B L]] | + | [[Category: Iverson BL]] |
| - | [[Category: Leysath, C E]] | + | [[Category: Leysath CE]] |
| - | [[Category: Monzingo, A F]] | + | [[Category: Monzingo AF]] |
| - | [[Category: Robertus, J D]] | + | [[Category: Robertus JD]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin]]
| + | |
| - | [[Category: Monoclonal antibody]]
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| - | [[Category: Single-chain fv]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The virulence of Bacillus anthracis is critically dependent on the cytotoxic components of the anthrax toxin, lethal factor (LF) and edema factor (EF). LF and EF gain entry into host cells through interactions with the protective antigen (PA), which binds to host cellular receptors such as CMG2. Antibodies that neutralize PA have been shown to confer protection in animal models and are undergoing intense clinical development. A murine monoclonal antibody, 14B7, has been reported to interact with domain 4 of PA (PAD4) and block its binding to CMG2. More recently, the 14B7 antibody was used as the platform for the selection of very high affinity, single-chain antibodies that have tremendous potential as a combination anthrax prophylactic and treatment. Here, we report the high-resolution X-ray structures of three high-affinity, single-chain antibodies in the 14B7 family; 14B7 and two high-affinity variants 1H and M18. In addition, we present the first neutralizing antibody-PA structure, M18 in complex with PAD4 at 3.8 A resolution. These structures provide insights into the mechanism of neutralization, and the effect of various mutations on antibody affinity, and enable a comparison between the binding of the M18 antibody and CMG2 with PAD4.
Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen.,Leysath CE, Monzingo AF, Maynard JA, Barnett J, Georgiou G, Iverson BL, Robertus JD J Mol Biol. 2009 Apr 3;387(3):680-93. Epub 2009 Feb 10. PMID:19361425[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Leysath CE, Monzingo AF, Maynard JA, Barnett J, Georgiou G, Iverson BL, Robertus JD. Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen. J Mol Biol. 2009 Apr 3;387(3):680-93. Epub 2009 Feb 10. PMID:19361425 doi:http://dx.doi.org/10.1016/j.jmb.2009.02.003
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