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| | <StructureSection load='3g7c' size='340' side='right'caption='[[3g7c]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='3g7c' size='340' side='right'caption='[[3g7c]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3g7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G7C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3g7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G7C FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wpa|1wpa]], [[1xaw|1xaw]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OCLN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7c OCA], [https://pdbe.org/3g7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g7c RCSB], [https://www.ebi.ac.uk/pdbsum/3g7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7c ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7c OCA], [https://pdbe.org/3g7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g7c RCSB], [https://www.ebi.ac.uk/pdbsum/3g7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g7c ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN]] Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:[https://omim.org/entry/251290 251290]]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.<ref>PMID:20727516</ref>
| + | [https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN] Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:[https://omim.org/entry/251290 251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.<ref>PMID:20727516</ref> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN]] May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.<ref>PMID:19114660</ref>
| + | [https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN] May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.<ref>PMID:19114660</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Antonetii, D A]] | + | [[Category: Antonetii DA]] |
| - | [[Category: Bewley, M C]] | + | [[Category: Bewley MC]] |
| - | [[Category: Flanagan, J M]] | + | [[Category: Flanagan JM]] |
| - | [[Category: Murakami, T]] | + | [[Category: Murakami T]] |
| - | [[Category: Sundstrom, J M]] | + | [[Category: Sundstrom JM]] |
| - | [[Category: Tash, B R]] | + | [[Category: Tash BR]] |
| - | [[Category: Adhesion]]
| + | |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Cell junction]]
| + | |
| - | [[Category: Coiled coil]]
| + | |
| - | [[Category: Diabetic retinopathy]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Occludin]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Tight junction]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Zo-1]]
| + | |
| Structural highlights
Disease
OCLN_HUMAN Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.[1]
Function
OCLN_HUMAN May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.[2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The molecular function of occludin, an integral membrane component of tight junctions, remains unclear. VEGF-induced phosphorylation sites were mapped on occludin by combining MS data analysis with bioinformatics. In vivo phosphorylation of Ser490 was validated and protein interaction studies combined with crystal structure analysis suggest that Ser490 phosphorylation attenuates the interaction between occludin and ZO-1. This study demonstrates that combining MS data and bioinformatics can successfully identify novel phosphorylation sites from limiting samples.
Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach.,Sundstrom JM, Tash BR, Murakami T, Flanagan JM, Bewley MC, Stanley BA, Gonsar KB, Antonetti DA J Proteome Res. 2009 Feb;8(2):808-17. PMID:19125584[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ O'Driscoll MC, Daly SB, Urquhart JE, Black GC, Pilz DT, Brockmann K, McEntagart M, Abdel-Salam G, Zaki M, Wolf NI, Ladda RL, Sell S, D'Arrigo S, Squier W, Dobyns WB, Livingston JH, Crow YJ. Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria. Am J Hum Genet. 2010 Sep 10;87(3):354-64. doi: 10.1016/j.ajhg.2010.07.012. Epub, 2010 Aug 19. PMID:20727516 doi:10.1016/j.ajhg.2010.07.012
- ↑ Suzuki T, Elias BC, Seth A, Shen L, Turner JR, Giorgianni F, Desiderio D, Guntaka R, Rao R. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):61-6. doi: 10.1073/pnas.0802741106., Epub 2008 Dec 29. PMID:19114660 doi:10.1073/pnas.0802741106
- ↑ Sundstrom JM, Tash BR, Murakami T, Flanagan JM, Bewley MC, Stanley BA, Gonsar KB, Antonetti DA. Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach. J Proteome Res. 2009 Feb;8(2):808-17. PMID:19125584 doi:10.1021/pr7007913
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