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| | <StructureSection load='3ial' size='340' side='right'caption='[[3ial]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='3ial' size='340' side='right'caption='[[3ial]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ial]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giaic Giaic]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IAL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ial]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IAL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PR8:5-O-[(R)-HYDROXY{[(2S)-PYRROLIDIN-2-YLCARBONYL]OXY}PHOSPHORYL]ADENOSINE'>PR8</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GL50803_15983, ProRS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=184922 GIAIC])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PR8:5-O-[(R)-HYDROXY{[(2S)-PYRROLIDIN-2-YLCARBONYL]OXY}PHOSPHORYL]ADENOSINE'>PR8</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Proline--tRNA_ligase Proline--tRNA ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.15 6.1.1.15] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ial FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ial OCA], [https://pdbe.org/3ial PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ial RCSB], [https://www.ebi.ac.uk/pdbsum/3ial PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ial ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ial FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ial OCA], [https://pdbe.org/3ial PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ial RCSB], [https://www.ebi.ac.uk/pdbsum/3ial PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ial ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A8BR89_GIAIC A8BR89_GIAIC] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Giaic]] | + | [[Category: Giardia lamblia ATCC 50803]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Proline--tRNA ligase]]
| + | [[Category: Larson ET]] |
| - | [[Category: Larson, E T]] | + | |
| | [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| - | [[Category: Merritt, E A]] | + | [[Category: Merritt EA]] |
| - | [[Category: Aar]]
| + | |
| - | [[Category: Aminoacyl-trna synthetase]]
| + | |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Cysr]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Msgpp]]
| + | |
| - | [[Category: Nucleotide-binding]]
| + | |
| - | [[Category: Pror]]
| + | |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Trna ligase]]
| + | |
| Structural highlights
Function
A8BR89_GIAIC
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The genome of the human intestinal parasite Giardia lamblia contains only a single aminoacyl-tRNA synthetase gene for each amino acid. The Giardia prolyl-tRNA synthetase gene product was originally misidentified as a dual-specificity Pro/Cys enzyme, in part owing to its unexpectedly high off-target activation of cysteine, but is now believed to be a normal representative of the class of archaeal/eukaryotic prolyl-tRNA synthetases. The 2.2 A resolution crystal structure of the G. lamblia enzyme presented here is thus the first structure determination of a prolyl-tRNA synthetase from a eukaryote. The relative occupancies of substrate (proline) and product (prolyl-AMP) in the active site are consistent with half-of-the-sites reactivity, as is the observed biphasic thermal denaturation curve for the protein in the presence of proline and MgATP. However, no corresponding induced asymmetry is evident in the structure of the protein. No thermal stabilization is observed in the presence of cysteine and ATP. The implied low affinity for the off-target activation product cysteinyl-AMP suggests that translational fidelity in Giardia is aided by the rapid release of misactivated cysteine.
Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia.,Larson ET, Kim JE, Napuli AJ, Verlinde CL, Fan E, Zucker FH, Van Voorhis WC, Buckner FS, Hol WG, Merritt EA Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1194-200. doi:, 10.1107/S0907444912024699. Epub 2012 Aug 18. PMID:22948920[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Larson ET, Kim JE, Napuli AJ, Verlinde CL, Fan E, Zucker FH, Van Voorhis WC, Buckner FS, Hol WG, Merritt EA. Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia. Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1194-200. doi:, 10.1107/S0907444912024699. Epub 2012 Aug 18. PMID:22948920 doi:http://dx.doi.org/10.1107/S0907444912024699
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