3igc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:47, 6 September 2023) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='3igc' size='340' side='right'caption='[[3igc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='3igc' size='340' side='right'caption='[[3igc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3igc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Varv Varv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGC FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3igc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Variola_virus Variola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGC FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">H6R, I6R, TOP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10255 VARV])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3igc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igc OCA], [https://pdbe.org/3igc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3igc RCSB], [https://www.ebi.ac.uk/pdbsum/3igc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3igc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3igc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igc OCA], [https://pdbe.org/3igc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3igc RCSB], [https://www.ebi.ac.uk/pdbsum/3igc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3igc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/TOP1_VAR67 TOP1_VAR67]] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
+
[https://www.uniprot.org/uniprot/TOP1_VAR67 TOP1_VAR67] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 37: Line 36:
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: DNA topoisomerase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Varv]]
+
[[Category: Variola virus]]
-
[[Category: Bushman, F D]]
+
[[Category: Bushman FD]]
-
[[Category: Duyne, G D.Van]]
+
[[Category: Hwang Y]]
-
[[Category: Hwang, Y]]
+
[[Category: Perry K]]
-
[[Category: Perry, K]]
+
[[Category: Van Duyne GD]]
-
[[Category: Atp-binding]]
+
-
[[Category: Dna-binding]]
+
-
[[Category: Isomerase]]
+
-
[[Category: Isomerase-dna complex]]
+
-
[[Category: Late protein]]
+
-
[[Category: Nucleotide-binding]]
+
-
[[Category: Poxvirus]]
+
-
[[Category: Protein-dna complex]]
+
-
[[Category: Topoisomerase]]
+

Current revision

Smallpox virus topoisomerase-DNA transition state

PDB ID 3igc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3igc"
Personal tools