3ipr

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the Enterococcus faecalis gluconate specific EIIA phosphotransferase system component

Structural highlights

3ipr is a 6 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q82ZC8_ENTFA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In Eubacteria, the utilization of a number of extracellular carbohydrates is mediated by sugar specific phosphoenolepyruvate (PEP) dependent sugar phosphotransferase systems (PTSs), which simultaneously import und phosphorylate their target sugars. Here, we report the crystal structure of the EIIA(gnt) component of the so far little investigated Enterococcus faecalis gluconate specific PTS. The crystal structure shows a tightly interacting dimer of EIIA(gnt) which is structurally similar to the related EIIA(man) from Escherichia coli. Homology modeling of E. faecalis HPr, EIIB(man) and their complexes with EIIA(man) suggests that despite moderate sequence identity between EIIA(man) and EIIA(gnt), the active sites closely match the situation observed in the E. coli system with His-9 of EIIA(gnt) being the likely phosphoryl group carrier. We therefore propose that the phosphoryl transfer reactions involving EIIA(gnt) proceed according to a mechanism analog to the one described for E. coli EIIA(man).

Structure of the Enterococcus faecalis EIIA(gnt) PTS component.,Reinelt S, Koch B, Hothorn M, Hengstenberg W, Welti S, Scheffzek K Biochem Biophys Res Commun. 2009 Oct 30;388(4):626-9. Epub 2009 Aug 13. PMID:19682976^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reinelt S, Koch B, Hothorn M, Hengstenberg W, Welti S, Scheffzek K. Structure of the Enterococcus faecalis EIIA(gnt) PTS component. Biochem Biophys Res Commun. 2009 Oct 30;388(4):626-9. Epub 2009 Aug 13. PMID:19682976 doi:10.1016/j.bbrc.2009.08.054

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ipr"

Categories: Enterococcus faecalis | Large Structures | Reinelt S | Scheffzek K | Welti S

@@ Line 3: / Line 3: @@
 <StructureSection load='3ipr' size='340' side='right'caption='[[3ipr]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ipr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IPR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ipr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IPR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EF_3136, gntF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ipr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ipr OCA], [https://pdbe.org/3ipr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ipr RCSB], [https://www.ebi.ac.uk/pdbsum/3ipr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ipr ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q82ZC8_ENTFA Q82ZC8_ENTFA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
+[[Category: Enterococcus faecalis]]
 [[Category: Large Structures]]
-[[Category: Reinelt, S]]
+[[Category: Reinelt S]]
-[[Category: Scheffzek, K]]
+[[Category: Scheffzek K]]
-[[Category: Welti, S]]
+[[Category: Welti S]]
-[[Category: Stranded parallel beta-sheet flanked by 3 alpha-helices on each side]]
-[[Category: Transferase]]

3ipr

From Proteopedia

Current revision

Crystal structure of the Enterococcus faecalis gluconate specific EIIA phosphotransferase system component

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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