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| | <StructureSection load='3k6m' size='340' side='right'caption='[[3k6m]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='3k6m' size='340' side='right'caption='[[3k6m]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k6m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3K6M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k6m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K6M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1o9l|1o9l]], [[1m3e|1m3e]], [[1ooy|1ooy]], [[1ooz|1ooz]], [[1ope|1ope]], [[2nrb|2nrb]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacid_CoA-transferase 3-oxoacid CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.5 2.8.3.5] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k6m OCA], [https://pdbe.org/3k6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k6m RCSB], [https://www.ebi.ac.uk/pdbsum/3k6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k6m ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3k6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k6m OCA], [http://pdbe.org/3k6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k6m RCSB], [http://www.ebi.ac.uk/pdbsum/3k6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k6m ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SCOT1_PIG SCOT1_PIG]] Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. | + | [https://www.uniprot.org/uniprot/SCOT1_PIG SCOT1_PIG] Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 3-oxoacid CoA-transferase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Sus scrofa]] | | [[Category: Sus scrofa]] |
| - | [[Category: Coker, A R]] | + | [[Category: Coker AR]] |
| - | [[Category: Coker, S]] | + | [[Category: Coker S]] |
| - | [[Category: Lewis, G R]] | + | [[Category: Lewis GR]] |
| - | [[Category: Lloyd, A]] | + | [[Category: Lloyd A]] |
| - | [[Category: Mitchell, E]] | + | [[Category: Mitchell E]] |
| - | [[Category: Shoolingin-Jordan, P]] | + | [[Category: Shoolingin-Jordan P]] |
| - | [[Category: Coa transferase]]
| + | |
| - | [[Category: Dynamic domain]]
| + | |
| - | [[Category: Glycerol]]
| + | |
| - | [[Category: Mitochondrion]]
| + | |
| - | [[Category: Scot]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transit peptide]]
| + | |
| Structural highlights
Function
SCOT1_PIG Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The enzyme succinyl-CoA:3-oxoacid coenzyme A transferase (SCOT) participates in the metabolism of ketone bodies in extrahepatic tissues. It catalyses the transfer of coenzyme A (CoA) from succinyl-CoA to acetoacetate with a classical ping-pong mechanism. There is biochemical evidence that the enzyme undergoes conformational changes during the reaction, but no domain movements have been reported in the available crystal structures. Here, a structure of pig heart SCOT refined at 1.5 A resolution is presented, showing that one of the four enzyme subunits in the crystallographic asymmetric unit has a molecule of glycerol bound in the active site; the glycerol molecule is hydrogen bonded to the conserved catalytic glutamate residue and is likely to occupy the cosubstrate-binding site. The binding of glycerol is associated with a substantial relative movement (a 13 degrees rotation) of two previously undefined domains that close around the substrate-binding site. The binding orientation of one of the cosubstrates, acetoacetate, is suggested based on the glycerol binding and the possibility that this dynamic domain movement is of functional importance is discussed.
The high-resolution structure of pig heart succinyl-CoA:3-oxoacid coenzyme A transferase.,Coker SF, Lloyd AJ, Mitchell E, Lewis GR, Coker AR, Shoolingin-Jordan PM Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):797-805. Epub 2010 Jun 19. PMID:20606260[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Coker SF, Lloyd AJ, Mitchell E, Lewis GR, Coker AR, Shoolingin-Jordan PM. The high-resolution structure of pig heart succinyl-CoA:3-oxoacid coenzyme A transferase. Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):797-805. Epub 2010 Jun 19. PMID:20606260 doi:10.1107/S0907444910018366
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