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| | <StructureSection load='3kml' size='340' side='right'caption='[[3kml]], [[Resolution|resolution]] 3.01Å' scene=''> | | <StructureSection load='3kml' size='340' side='right'caption='[[3kml]], [[Resolution|resolution]] 3.01Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3kml]] is a 17 chain structure with sequence from [https://en.wikipedia.org/wiki/Tmv Tmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KML FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3kml]] is a 17 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus_(vulgare) Tobacco mosaic virus (vulgare)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KML FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12243 TMV])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.011Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kml OCA], [https://pdbe.org/3kml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kml RCSB], [https://www.ebi.ac.uk/pdbsum/3kml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kml ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kml OCA], [https://pdbe.org/3kml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kml RCSB], [https://www.ebi.ac.uk/pdbsum/3kml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kml ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/CAPSD_TMV CAPSD_TMV]] Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
| + | [https://www.uniprot.org/uniprot/CAPSD_TMV CAPSD_TMV] Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tmv]]
| + | [[Category: Berger JM]] |
| - | [[Category: Berger, J M]] | + | [[Category: Dedeo MT]] |
| - | [[Category: Dedeo, M T]] | + | [[Category: Duderstadt KE]] |
| - | [[Category: Duderstadt, K E]] | + | [[Category: Francis MB]] |
| - | [[Category: Francis, M B]] | + | |
| - | [[Category: Acetylation]]
| + | |
| - | [[Category: Capsid protein]]
| + | |
| - | [[Category: Cptmvp]]
| + | |
| - | [[Category: Permutant]]
| + | |
| - | [[Category: Tmvp]]
| + | |
| - | [[Category: Tobacco mosaic virus coat protein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Virion]]
| + | |
| Structural highlights
Function
CAPSD_TMV Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The protein coat of the tobacco mosaic virus (TMV) has been explored extensively for the construction of nanoscale architectures. In previous work, we have reported efficient TMV-based light harvesting systems bearing chromophores in a hollow channel of the assembled protein. We have also reported an N-terminal transamination/oximation method that could be used to attach electrodes and catalytic groups to the exterior surface of the rods. To complement these techniques, we report herein a new circular permutant of the TMV capsid protein that repositions the N- and C-termini to the center of the assemblies. This protein can be produced in very high yield through E. coli expression and self-assembles into light harvesting rods that are much like those assembled from the wild-type protein. However, the disks formed from the permutant structure are stable over a significantly wider pH range, greatly improving the practicality of this assembled form for materials applications. The new position of the N-terminus allows functional groups to be installed in the inner pore of the disks, affording geometries reminiscent of natural photosynthetic systems. The permutant also shows the ability to coassemble with regular monomers, allowing the future generation of multicomponent rod structures that are modified on the exterior and interior surfaces, as well as in the internal RNA channel.
Nanoscale protein assemblies from a circular permutant of the tobacco mosaic virus.,Dedeo MT, Duderstadt KE, Berger JM, Francis MB Nano Lett. 2010 Jan;10(1):181-6. PMID:19924865[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Dedeo MT, Duderstadt KE, Berger JM, Francis MB. Nanoscale protein assemblies from a circular permutant of the tobacco mosaic virus. Nano Lett. 2010 Jan;10(1):181-6. PMID:19924865 doi:10.1021/nl9032395
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