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| | ==Crystal structure of Leishmania mexicana pyruvate kinase (LmPYK)in complex with 1,3,6,8-pyrenetetrasulfonic acid== | | ==Crystal structure of Leishmania mexicana pyruvate kinase (LmPYK)in complex with 1,3,6,8-pyrenetetrasulfonic acid== |
| - | <StructureSection load='3ktx' size='340' side='right' caption='[[3ktx]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3ktx' size='340' side='right'caption='[[3ktx]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ktx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leime Leime]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ktx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_mexicana Leishmania mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KTX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PTK:PYRENE-1,3,6,8-TETRASULFONIC+ACID'>PTK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pkl|1pkl]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PTK:PYRENE-1,3,6,8-TETRASULFONIC+ACID'>PTK</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5665 LEIME])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ktx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktx OCA], [https://pdbe.org/3ktx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ktx RCSB], [https://www.ebi.ac.uk/pdbsum/3ktx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktx ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktx OCA], [http://pdbe.org/3ktx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ktx RCSB], [http://www.ebi.ac.uk/pdbsum/3ktx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/KPYK_LEIME KPYK_LEIME] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Pyruvate Kinase|Pyruvate Kinase]] | + | *[[Pyruvate kinase 3D structures|Pyruvate kinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Leime]] | + | [[Category: Large Structures]] |
| - | [[Category: Pyruvate kinase]] | + | [[Category: Leishmania mexicana]] |
| - | [[Category: Morgan, H P]] | + | [[Category: Morgan HP]] |
| - | [[Category: Walkinshaw, M D]] | + | [[Category: Walkinshaw MD]] |
| - | [[Category: Allosteric enzyme]]
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| - | [[Category: Atp-binding]]
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| - | [[Category: Glycolysis]]
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| - | [[Category: Kinase]]
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| - | [[Category: Magnesium]]
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| - | [[Category: Metal-binding]]
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| - | [[Category: Nucleotide-binding]]
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| - | [[Category: Pyruvate]]
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| - | [[Category: Transferase]]
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| Structural highlights
Function
KPYK_LEIME
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The inclusion of novel small molecules in crystallization experiments has provided very encouraging results and this method is now emerging as a promising alternative strategy for crystallizing 'problematic' biological macromolecules. These small molecules have the ability to promote lattice formation through stabilizing intermolecular interactions in protein crystals. Here, the use of 1,3,6,8-pyrenetetrasulfonic acid (PTS), which provides a helpful intermolecular bridge between Leishmania mexicana PYK (LmPYK) macromolecules in the crystal, is reported, resulting in the rapid formation of a more stable crystal lattice at neutral pH and greatly improved X-ray diffraction results. The refined structure of the LmPYK-PTS complex revealed the negatively charged PTS molecule to be stacked between positively charged (surface-exposed) arginine side chains from neighbouring LmPYK molecules in the crystal lattice.
An improved strategy for the crystallization of Leishmania mexicana pyruvate kinase.,Morgan HP, McNae IW, Hsin KY, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt, 3):215-8. Epub 2010 Feb 23. PMID:20208146[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Morgan HP, McNae IW, Hsin KY, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD. An improved strategy for the crystallization of Leishmania mexicana pyruvate kinase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt, 3):215-8. Epub 2010 Feb 23. PMID:20208146 doi:10.1107/S1744309109053494
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