1mqk
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1mqk.jpg|left|200px]] | [[Image:1mqk.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1mqk", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1mqk| PDB=1mqk | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2''' | '''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2''' | ||
| Line 29: | Line 26: | ||
[[Category: Michel, H.]] | [[Category: Michel, H.]] | ||
[[Category: Ostermeier, C.]] | [[Category: Ostermeier, C.]] | ||
| - | [[Category: | + | [[Category: Antibody]] |
| - | [[Category: | + | [[Category: Cytochrome c oxidase]] |
| - | [[Category: | + | [[Category: High-resolution structure]] |
| - | [[Category: | + | [[Category: Membrane protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:36:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:36, 2 May 2008
Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2
Overview
The antibody Fv fragment 7E2 has previously been employed in the induced crystallization of the integral membrane protein cytochrome c oxidase from Paracoccus denitrificans. The 1.3 A X-ray structure of the uncomplexed antibody fragment reveals conserved water networks on the surfaces of the framework regions. A novel consensus motif for water coordination, XX(S/T), is found along the edges of the beta-sandwich, where a water molecule forms hydrogen bonds to the carbonyl O atom of a residue at position N and the OG hydroxyl groups of conserved serines or threonines at position N + 2. Multiple conformations were found in the hydrophobic core for residues IleL21, LeuL33 and the disulfide bridges. An internal water molecule that is compatible with only one of the three packing states of the V(L) core suggests local 'breathing' of the variable domain. TrpH47, a conserved key residue of the V(H)/V(L) interface, is crucially involved in the formation of the antigen-binding site by adopting a novel conformation that specifically stabilizes the non-canonical CDR-L3 loop. Finally, a comparison with 7E2-cytochrome c oxidase complexes demonstrates that binding of this membrane-bound antigen proceeds without major conformational changes of the 7E2 antibody fragment.
About this Structure
1MQK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization., Essen LO, Harrenga A, Ostermeier C, Michel H, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):677-87. Epub 2003, Mar 25. PMID:12657787 Page seeded by OCA on Sat May 3 01:36:08 2008
