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| | ==L-arabinitol 4-dehydrogenase== | | ==L-arabinitol 4-dehydrogenase== |
| - | <StructureSection load='3m6i' size='340' side='right' caption='[[3m6i]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3m6i' size='340' side='right'caption='[[3m6i]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3m6i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M6I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3m6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M6I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NCU00643, NCU00643.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Chrysonilia crassa])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-arabinitol_4-dehydrogenase L-arabinitol 4-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.12 1.1.1.12] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6i OCA], [https://pdbe.org/3m6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m6i RCSB], [https://www.ebi.ac.uk/pdbsum/3m6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m6i ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6i OCA], [http://pdbe.org/3m6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m6i RCSB], [http://www.ebi.ac.uk/pdbsum/3m6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m6i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LAD_NEUCR LAD_NEUCR]] Catalyzes the NAD-dependent oxidation of L-arabinitol to L-xylulose in the fungal L-arabinose catabolic pathway. L-arabinose catabolism is important for using plant material as a carbon source. Not active on D-arabinitol, D-sorbitol and D-mannitol.<ref>PMID:17938906</ref> | + | [https://www.uniprot.org/uniprot/LAD_NEUCR LAD_NEUCR] Catalyzes the NAD-dependent oxidation of L-arabinitol to L-xylulose in the fungal L-arabinose catabolic pathway. L-arabinose catabolism is important for using plant material as a carbon source. Not active on D-arabinitol, D-sorbitol and D-mannitol.<ref>PMID:17938906</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chrysonilia crassa]] | + | [[Category: Large Structures]] |
| - | [[Category: L-arabinitol 4-dehydrogenase]] | + | [[Category: Neurospora crassa]] |
| - | [[Category: Bae, B]] | + | [[Category: Bae B]] |
| - | [[Category: Nair, S K]] | + | [[Category: Nair SK]] |
| - | [[Category: Medium chain dehydrogenase/reductase]]
| + | |
| - | [[Category: Oxidoreductase]]
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| Structural highlights
Function
LAD_NEUCR Catalyzes the NAD-dependent oxidation of L-arabinitol to L-xylulose in the fungal L-arabinose catabolic pathway. L-arabinose catabolism is important for using plant material as a carbon source. Not active on D-arabinitol, D-sorbitol and D-mannitol.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
L-arabinitol 4-dehydrogenase (LAD) catalyzes the conversion of l-arabinitol into l-xylulose with concomitant NAD(+) reduction. It is an essential enzyme in the development of recombinant organisms that convert l-arabinose into fuels and chemicals using the fungal l-arabinose catabolic pathway. Here we report the crystal structure of LAD from the filamentous fungus Neurospora crassa at 2.6 A resolution. In addition, we created a number of site-directed variants of N. crassa LAD that are capable of utilizing NADP(+) as cofactor, yielding the first example of LAD with an almost completely switched cofactor specificity. This work represents the first structural data on any LAD and provides a molecular basis for understanding the existing literature on the substrate specificity and cofactor specificity of this enzyme. The engineered LAD mutants with altered cofactor specificity should be useful for applications in industrial biotechnology.
Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa.,Bae B, Sullivan RP, Zhao H, Nair SK J Mol Biol. 2010 Sep 10;402(1):230-40. Epub 2010 Jul 22. PMID:20655316[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sullivan R, Zhao H. Cloning, characterization, and mutational analysis of a highly active and stable L-arabinitol 4-dehydrogenase from Neurospora crassa. Appl Microbiol Biotechnol. 2007 Dec;77(4):845-52. Epub 2007 Oct 16. PMID:17938906 doi:10.1007/s00253-007-1225-0
- ↑ Bae B, Sullivan RP, Zhao H, Nair SK. Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa. J Mol Biol. 2010 Sep 10;402(1):230-40. Epub 2010 Jul 22. PMID:20655316 doi:10.1016/j.jmb.2010.07.033
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