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| | ==Ets1 complex with stromelysin-1 promoter DNA== | | ==Ets1 complex with stromelysin-1 promoter DNA== |
| - | <StructureSection load='3mfk' size='340' side='right' caption='[[3mfk]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='3mfk' size='340' side='right'caption='[[3mfk]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3mfk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MFK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3mfk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MFK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ETS1, EWSR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mfk OCA], [http://pdbe.org/3mfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mfk RCSB], [http://www.ebi.ac.uk/pdbsum/3mfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mfk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mfk OCA], [https://pdbe.org/3mfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mfk RCSB], [https://www.ebi.ac.uk/pdbsum/3mfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mfk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN]] Transcription factor.<ref>PMID:10698492</ref> | + | [https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN] Transcription factor.<ref>PMID:10698492</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Babayeva, N D]] | + | [[Category: Large Structures]] |
| - | [[Category: Mino, K]] | + | [[Category: Babayeva ND]] |
| - | [[Category: Tahirov, T H]] | + | [[Category: Mino K]] |
| - | [[Category: Ets domain]] | + | [[Category: Tahirov TH]] |
| - | [[Category: Ets1]]
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| - | [[Category: Protein-dna complex]]
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| - | [[Category: Stromelysin-1]]
| + | |
| - | [[Category: Transcription-dna complex]]
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| Structural highlights
Function
ETS1_HUMAN Transcription factor.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ets1 is a member of the Ets family of transcription factors. Ets1 is autoinhibited and its activation requires heterodimerization with a partner protein or DNA-mediated homodimerization for cooperative DNA binding. In the latter case, Ets1 molecules bind to palindromic sequences in which two Ets-binding sites (EBS) are separated by four base pairs, for example in the promoters of stromelysin-1 and p53. Interestingly, counteraction of autoinhibition requires the autoinhibitory region encoded by exon VII of the gene. The structural basis for the requirement of autoinhibitory sequences for Ets1 binding to palindromic EBS still remains unresolved. Here we report the crystal structure of two Ets1 molecules bound to an EBS palindrome of the stromelysin-1 promoter DNA, providing a plausible explanation for the requirement of exon VII-encoded sequences for Ets1 cooperative DNA binding. The proposed mechanism was verified both in vitro by surface plasmon resonance and in vivo by transcription-based assays.
Structural basis of Ets1 cooperative binding to palindromic sequences on stromelysin-1 promoter DNA.,Babayeva ND, Wilder PJ, Shiina M, Mino K, Desler M, Ogata K, Rizzino A, Tahirov TH Cell Cycle. 2010 Aug;9(15):3054-62. Epub 2010 Aug 5. PMID:20686355[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li R, Pei H, Watson DK, Papas TS. EAP1/Daxx interacts with ETS1 and represses transcriptional activation of ETS1 target genes. Oncogene. 2000 Feb 10;19(6):745-53. PMID:10698492 doi:10.1038/sj.onc.1203385
- ↑ Babayeva ND, Wilder PJ, Shiina M, Mino K, Desler M, Ogata K, Rizzino A, Tahirov TH. Structural basis of Ets1 cooperative binding to palindromic sequences on stromelysin-1 promoter DNA. Cell Cycle. 2010 Aug;9(15):3054-62. Epub 2010 Aug 5. PMID:20686355
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