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| | ==PduA from Salmonella enterica Typhimurium== | | ==PduA from Salmonella enterica Typhimurium== |
| - | <StructureSection load='3ngk' size='340' side='right' caption='[[3ngk]], [[Resolution|resolution]] 2.26Å' scene=''> | + | <StructureSection load='3ngk' size='340' side='right'caption='[[3ngk]], [[Resolution|resolution]] 2.26Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ngk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NGK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ngk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NGK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pduA, STM2038 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2608Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ngk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngk OCA], [http://pdbe.org/3ngk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ngk RCSB], [http://www.ebi.ac.uk/pdbsum/3ngk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ngk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ngk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngk OCA], [https://pdbe.org/3ngk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ngk RCSB], [https://www.ebi.ac.uk/pdbsum/3ngk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ngk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PDUA_SALTY PDUA_SALTY] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus typhimurium loeffler 1892]] | + | [[Category: Large Structures]] |
| - | [[Category: Cascio, D]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Crowley, C S]] | + | [[Category: Cascio D]] |
| - | [[Category: Sawaya, M R]] | + | [[Category: Crowley CS]] |
| - | [[Category: Yeates, T O]] | + | [[Category: Sawaya MR]] |
| - | [[Category: Bmc shell protein]] | + | [[Category: Yeates TO]] |
| - | [[Category: Carboxysome]]
| + | |
| - | [[Category: Pdu]]
| + | |
| - | [[Category: Propanediol]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
PDUA_SALTY
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Bacterial microcompartments are a functionally diverse group of proteinaceous organelles that confine specific reaction pathways in the cell within a thin protein-based shell. The propanediol utilizing (Pdu) microcompartment contains the reactions for metabolizing 1,2-propanediol in certain enteric bacteria, including Salmonella. The Pdu shell is assembled from a few thousand protein subunits of several different types. Here we report the crystal structures of two key shell proteins, PduA and PduT. The crystal structures offer insights into the mechanisms of Pdu microcompartment assembly and molecular transport across the shell. PduA forms a symmetric homohexamer whose central pore appears tailored for facilitating transport of the 1,2-propanediol substrate. PduT is a novel, tandem domain shell protein that assembles as a pseudohexameric homotrimer. Its structure reveals an unexpected site for binding an [Fe-S] cluster at the center of the PduT pore. The location of a metal redox cofactor in the pore of a shell protein suggests a novel mechanism for either transferring redox equivalents across the shell or for regenerating luminal [Fe-S] clusters.
Structural insights into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell.,Crowley CS, Cascio D, Sawaya MR, Kopstein JS, Bobik TA, Yeates TO J Biol Chem. 2010 Sep 24. PMID:20870711[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Crowley CS, Cascio D, Sawaya MR, Kopstein JS, Bobik TA, Yeates TO. Structural insights into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell. J Biol Chem. 2010 Sep 24. PMID:20870711 doi:10.1074/jbc.M110.160580
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