3ob4
From Proteopedia
(Difference between revisions)
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==MBP-fusion protein of the major peanut allergen Ara h 2== | ==MBP-fusion protein of the major peanut allergen Ara h 2== | ||
| - | <StructureSection load='3ob4' size='340' side='right'caption='[[3ob4]]' scene=''> | + | <StructureSection load='3ob4' size='340' side='right'caption='[[3ob4]], [[Resolution|resolution]] 2.71Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OB4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ob4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OB4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ob4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ob4 OCA], [https://pdbe.org/3ob4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ob4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ob4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ob4 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.706Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ob4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ob4 OCA], [https://pdbe.org/3ob4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ob4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ob4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ob4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/A5Z1Q8_ARADU A5Z1Q8_ARADU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BACKGROUND: Peanut allergy affects 1% of the population and causes the most fatal food-related anaphylactic reactions. The protein Ara h 2 is the most potent peanut allergen recognized by 80-90% of peanut allergic patients. METHODS: The crystal structure of the major peanut allergen Ara h 2 was determined for the first time at 2.7 A resolution using a customized maltose-binding protein (MBP)-fusion system. IgE antibody binding to the MBP fusion construct vs the natural allergen was compared by ELISA using sera from peanut allergic patients. RESULTS: The structure of Ara h 2 is a five-helix bundle held together by four disulfide bonds and related to the prolamin protein superfamily. The fold is most similar to other amylase and trypsin inhibitors. The MBP--Ara h 2 fusion construct was positively recognized by IgE from 76% of allergic patients (25/33). Two populations of patients could be identified. Subpopulation 1 (n = 14) showed an excellent correlation of IgE antibody binding to natural vs recombinant Ara h 2. Subpopulation 2 (n = 15) showed significantly reduced IgE binding to the MBP fusion protein. Interestingly, about 20% of the IgE binding in subpopulation 2 could be recovered by increasing the distance between MBP and Ara h 2 in a second construct. DISCUSSION: The reduced IgE binding to the MBP--Ara h 2 of subpopulation 2 indicates that the MBP molecule protects an immunodominant epitope region near the first helix of Ara h 2. Residues involved in the epitope(s) are suggested by the crystal structure. The MBP--Ara h 2 fusion constructs will be useful to further elucidate the relevance of certain epitopes to peanut allergy. | ||
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| + | Ara h 2: crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversity.,Mueller GA, Gosavi RA, Pomes A, Wunschmann S, Moon AF, London RE, Pedersen LC Allergy. 2011 Jul;66(7):878-85. doi: 10.1111/j.1398-9995.2010.02532.x. Epub 2011 , Jan 21. PMID:21255036<ref>PMID:21255036</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3ob4" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]] | *[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arachis hypogaea]] | ||
| + | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Gosavi RA]] | [[Category: Gosavi RA]] | ||
Revision as of 09:32, 6 September 2023
MBP-fusion protein of the major peanut allergen Ara h 2
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