|
|
| Line 3: |
Line 3: |
| | <StructureSection load='3owg' size='340' side='right'caption='[[3owg]], [[Resolution|resolution]] 2.86Å' scene=''> | | <StructureSection load='3owg' size='340' side='right'caption='[[3owg]], [[Resolution|resolution]] 2.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3owg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccw Vaccw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OWG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3owg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OWG FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ga9|2ga9]], [[3er9|3er9]], [[3er8|3er8]], [[3erc|3erc]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.86Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PAPL, VACWR057, E1L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10254 VACCW])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3owg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owg OCA], [https://pdbe.org/3owg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3owg RCSB], [https://www.ebi.ac.uk/pdbsum/3owg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3owg ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3owg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owg OCA], [https://pdbe.org/3owg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3owg RCSB], [https://www.ebi.ac.uk/pdbsum/3owg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3owg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PAP1_VACCW PAP1_VACCW]] Polymerase that creates the 3'-poly(A) tail of mRNA's.
| + | [https://www.uniprot.org/uniprot/PAP1_VACCW PAP1_VACCW] Polymerase that creates the 3'-poly(A) tail of mRNA's. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 20: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Poly(A) Polymerase|Poly(A) Polymerase]] | + | *[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 28: |
Line 26: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Polynucleotide adenylyltransferase]] | + | [[Category: Vaccinia virus WR]] |
| - | [[Category: Vaccw]]
| + | [[Category: Gershon PD]] |
| - | [[Category: Gershon, P D]] | + | [[Category: Li C]] |
| - | [[Category: Li, C]] | + | [[Category: Li H]] |
| - | [[Category: Li, H]] | + | [[Category: Poulos TL]] |
| - | [[Category: Poulos, T L]] | + | [[Category: Zhou S]] |
| - | [[Category: Zhou, S]] | + | |
| - | [[Category: Polyadenylate polymerase]]
| + | |
| - | [[Category: Rna polyadenylate polymerase complex]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Translocation]]
| + | |
| Structural highlights
Function
PAP1_VACCW Polymerase that creates the 3'-poly(A) tail of mRNA's.
Publication Abstract from PubMed
Vaccinia virus poly(A) polymerase (VP55) is the only known polymerase that can translocate independently with respect to single-stranded nucleic acid (ssNA). Previously, its structure has only been solved in the context of the VP39 processivity factor. Here, a crystal structure of unliganded monomeric VP55 has been solved to 2.86 A resolution, showing the first backbone structural isoforms among either VP55 or its processivity factor (VP39). Backbone differences between the two molecules of VP55 in the asymmetric unit indicated that unliganded monomeric VP55 can undergo a `rocking' motion of the N-terminal domain with respect to the other two domains, which may be `rigidified' upon VP39 docking. This observation is consistent with previously demonstrated experimental molecular dynamics of the monomer during translocation with respect to nucleic acid and with different mechanisms of translocation in the presence and absence of processivity factor VP39. Side-chain conformational changes in the absence of ligand were observed at a key primer contact site and at the catalytic center of VP55. The current structure completes the trio of possible structural forms for VP55 and VP39, namely the VP39 monomer, the VP39-VP55 heterodimer and the VP55 monomer.
Domain-level rocking motion within a polymerase that translocates on single-stranded nucleic acid.,Li H, Li C, Zhou S, Poulos TL, Gershon PD Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):617-24. doi:, 10.1107/S0907444913000346. Epub 2013 Mar 14. PMID:23519670[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li H, Li C, Zhou S, Poulos TL, Gershon PD. Domain-level rocking motion within a polymerase that translocates on single-stranded nucleic acid. Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):617-24. doi:, 10.1107/S0907444913000346. Epub 2013 Mar 14. PMID:23519670 doi:http://dx.doi.org/10.1107/S0907444913000346
|
