1msw
From Proteopedia
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'''Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase''' | '''Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase''' | ||
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[[Category: Steitz, T A.]] | [[Category: Steitz, T A.]] | ||
[[Category: Yin, Y W.]] | [[Category: Yin, Y W.]] | ||
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Revision as of 22:40, 2 May 2008
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase
Overview
To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a "transcription bubble" interacting with a 17-nucleotide RNA transcript. The transition from an initiation to an elongation complex is accompanied by a major refolding of the amino-terminal 300 residues. This results in loss of the promoter binding site, facilitating promoter clearance, and creates a tunnel that surrounds the RNA transcript after it peels off a seven-base pair heteroduplex. Formation of the exit tunnel explains the enhanced processivity of the elongation complex. Downstream duplex DNA binds to the fingers domain, and its orientation relative to upstream DNA in the initiation complex implies an unwinding that could facilitate formation of the open promoter complex.
About this Structure
1MSW is a Single protein structure of sequence from Enterobacteria phage t7. The following page contains interesting information on the relation of 1MSW with [RNA Polymerase]. Full crystallographic information is available from OCA.
Reference
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase., Yin YW, Steitz TA, Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:12242451 Page seeded by OCA on Sat May 3 01:40:53 2008
