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2cny

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Revision as of 10:26, 6 September 2023

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)

Structural highlights

2cny is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8ZRK4_SALTY

Publication Abstract from PubMed

Gram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled.

Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism.,Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G Mol Cell. 2006 Jun 23;22(6):831-42. PMID:16793551^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G. Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism. Mol Cell. 2006 Jun 23;22(6):831-42. PMID:16793551 doi:10.1016/j.molcel.2006.05.033

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cny"

@@ Line 3: / Line 3: @@
 <StructureSection load='2cny' size='340' side='right'caption='[[2cny]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cny]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cny]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNY FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2cnz|2cnz]], [[2co1|2co1]], [[2co2|2co2]], [[2co3|2co3]], [[2co4|2co4]], [[2co6|2co6]], [[2co7|2co7]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cny OCA], [https://pdbe.org/2cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cny RCSB], [https://www.ebi.ac.uk/pdbsum/2cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cny ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Ashcroft, A E]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-[[Category: Hannan, T J]]
+[[Category: Ashcroft AE]]
-[[Category: Hultgren, S J]]
+[[Category: Hannan TJ]]
-[[Category: Radford, S E]]
+[[Category: Hultgren SJ]]
-[[Category: Remaut, H]]
+[[Category: Radford SE]]
-[[Category: Rose, R J]]
+[[Category: Remaut H]]
-[[Category: Waksman, G]]
+[[Category: Rose RJ]]
-[[Category: Fibril protein]]
+[[Category: Waksman G]]
-[[Category: Fold complementation]]
-[[Category: Pilus subunit]]

2cny

From Proteopedia

Revision as of 10:26, 6 September 2023

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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