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| | <StructureSection load='5jcy' size='340' side='right'caption='[[5jcy]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='5jcy' size='340' side='right'caption='[[5jcy]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jcy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JCY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JCY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jcy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JCY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MYO5A, MYH12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jcy OCA], [http://pdbe.org/5jcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jcy RCSB], [http://www.ebi.ac.uk/pdbsum/5jcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jcy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jcy OCA], [https://pdbe.org/5jcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jcy RCSB], [https://www.ebi.ac.uk/pdbsum/5jcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jcy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref> [[http://www.uniprot.org/uniprot/SPIR2_HUMAN SPIR2_HUMAN]] Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.<ref>PMID:21620703</ref> | + | [https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Houdusse, A]] | + | [[Category: Houdusse A]] |
| - | [[Category: Kerkhoff, E]] | + | [[Category: Kerkhoff E]] |
| - | [[Category: Malherbes, G]] | + | [[Category: Malherbes G]] |
| - | [[Category: Pylypenko, O]] | + | [[Category: Pylypenko O]] |
| - | [[Category: Welz, T]] | + | [[Category: Welz T]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Myosin]]
| + | |
| - | [[Category: Spir actin nucleator]]
| + | |
| Structural highlights
Disease
MYO5A_HUMAN Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
Function
MYO5A_HUMAN Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.[1]
Publication Abstract from PubMed
There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment. The myosin V globular tail domain (MyoV-GTD) interacts directly with an evolutionarily conserved Spir sequence motif. We determined crystal structures of MyoVa-GTD bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form. The ternary complex architecture explains how Rab11 vesicles support coordinated F-actin nucleation and myosin force generation for vesicle transport and tethering. New insights are also provided into how myosin activation can be coupled with the generation of actin tracks. Since MyoV binds several Rab GTPases, synchronized nucleator and motor targeting could provide a common mechanism to control force generation and motility in different cellular processes.
Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.,Pylypenko O, Welz T, Tittel J, Kollmar M, Chardon F, Malherbe G, Weiss S, Michel CI, Samol-Wolf A, Grasskamp AT, Hume A, Goud B, Baron B, England P, Titus MA, Schwille P, Weidemann T, Houdusse A, Kerkhoff E Elife. 2016 Sep 13;5. pii: e17523. doi: 10.7554/eLife.17523. PMID:27623148[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Mehta AD, Rock RS, Rief M, Spudich JA, Mooseker MS, Cheney RE. Myosin-V is a processive actin-based motor. Nature. 1999 Aug 5;400(6744):590-3. PMID:10448864 doi:http://dx.doi.org/10.1038/23072
- ↑ Pylypenko O, Welz T, Tittel J, Kollmar M, Chardon F, Malherbe G, Weiss S, Michel CI, Samol-Wolf A, Grasskamp AT, Hume A, Goud B, Baron B, England P, Titus MA, Schwille P, Weidemann T, Houdusse A, Kerkhoff E. Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. Elife. 2016 Sep 13;5. pii: e17523. doi: 10.7554/eLife.17523. PMID:27623148 doi:http://dx.doi.org/10.7554/eLife.17523
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