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| | ==Geranylgeranyl Pyrophosphate Synthetase from archaeon Geoglobus acetivorans== | | ==Geranylgeranyl Pyrophosphate Synthetase from archaeon Geoglobus acetivorans== |
| - | <StructureSection load='5jfq' size='340' side='right' caption='[[5jfq]], [[Resolution|resolution]] 2.51Å' scene=''> | + | <StructureSection load='5jfq' size='340' side='right'caption='[[5jfq]], [[Resolution|resolution]] 2.51Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jfq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_21716 Dsm 21716]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JFQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jfq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geoglobus_acetivorans Geoglobus acetivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JFQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GACE_1337 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565033 DSM 21716])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfq OCA], [http://pdbe.org/5jfq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jfq RCSB], [http://www.ebi.ac.uk/pdbsum/5jfq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfq OCA], [https://pdbe.org/5jfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jfq RCSB], [https://www.ebi.ac.uk/pdbsum/5jfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GGPPS_GEOAI GGPPS_GEOAI] Catalyzes the addition of 3 molecules of isopentenyl diphosphate (IPP) onto dimethylallyl diphosphate (DMAPP) to form geranylgeranyl pyrophosphate (GGPP). Catalyzes the synthesis of geranylgeranyl pyrophosphate as a major product and of farnesyl pyrophosphate in smaller amounts.<ref>PMID:30062607</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dsm 21716]] | + | [[Category: Geoglobus acetivorans]] |
| - | [[Category: Boyko, K M]] | + | [[Category: Large Structures]] |
| - | [[Category: Mardanov, A V]] | + | [[Category: Boyko KM]] |
| - | [[Category: Nikolaeva, A Y]] | + | [[Category: Mardanov AV]] |
| - | [[Category: Petrova, T]] | + | [[Category: Nikolaeva AY]] |
| - | [[Category: Popov, V O]] | + | [[Category: Petrova T]] |
| - | [[Category: Rakitin, A L]] | + | [[Category: Popov VO]] |
| - | [[Category: Ravin, N V]] | + | [[Category: Rakitin AL]] |
| - | [[Category: Stekhanova, T N]] | + | [[Category: Ravin NV]] |
| - | [[Category: Archaeon]]
| + | [[Category: Stekhanova TN]] |
| - | [[Category: Geranylgeranyl pyrophosphate synthetase]]
| + | |
| - | [[Category: Hyperthermophilic]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
GGPPS_GEOAI Catalyzes the addition of 3 molecules of isopentenyl diphosphate (IPP) onto dimethylallyl diphosphate (DMAPP) to form geranylgeranyl pyrophosphate (GGPP). Catalyzes the synthesis of geranylgeranyl pyrophosphate as a major product and of farnesyl pyrophosphate in smaller amounts.[1]
Publication Abstract from PubMed
A novel type 1 geranylgeranyl pyrophosphate synthase GACE1337 has been identified within the genome of a newly identified hyperthermophilic archaeon Geoglobus acetivorans. The enzyme has been cloned and over-expressed in Escherichia coli. The recombinant enzyme has been biochemically and structurally characterized. It is able to catalyze the synthesis of geranylgeranyl pyrophosphate as a major product and of farnesyl pyrophosphate in smaller amounts, as measured by gas chromatography-mass spectrometry at an elevated temperature of 60 degrees C. Its ability to produce two products is consistent with the fact that GACE1337 is the only short-chain isoprenyl diphosphate synthase in G. acetivorans. Attempts to crystallize the enzyme were successful only at 37 degrees C. The three-dimensional structure of GACE1337 was determined by X-ray diffraction to 2.5 A resolution. A comparison of its structure with those of related enzymes revealed that the Geoglobus enzyme has the features of both type I and type III geranylgeranyl pyrophosphate synthases, which allow it to regulate the product length. The active enzyme is a dimer and has three aromatic amino acids, two Phe, and a Tyr, located in the hydrophobic cleft between the two subunits. It is proposed that these bulky residues play a major role in the synthetic reaction by controlling the product elongation.
Structural characterization of geranylgeranyl pyrophosphate synthase GACE1337 from the hyperthermophilic archaeon Geoglobus acetivorans.,Petrova TE, Boyko KM, Nikolaeva AY, Stekhanova TN, Gruzdev EV, Mardanov AV, Stroilov VS, Littlechild JA, Popov VO, Bezsudnova EY Extremophiles. 2018 Nov;22(6):877-888. doi: 10.1007/s00792-018-1044-5. Epub 2018 , Jul 30. PMID:30062607[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Petrova TE, Boyko KM, Nikolaeva AY, Stekhanova TN, Gruzdev EV, Mardanov AV, Stroilov VS, Littlechild JA, Popov VO, Bezsudnova EY. Structural characterization of geranylgeranyl pyrophosphate synthase GACE1337 from the hyperthermophilic archaeon Geoglobus acetivorans. Extremophiles. 2018 Nov;22(6):877-888. doi: 10.1007/s00792-018-1044-5. Epub 2018 , Jul 30. PMID:30062607 doi:http://dx.doi.org/10.1007/s00792-018-1044-5
- ↑ Petrova TE, Boyko KM, Nikolaeva AY, Stekhanova TN, Gruzdev EV, Mardanov AV, Stroilov VS, Littlechild JA, Popov VO, Bezsudnova EY. Structural characterization of geranylgeranyl pyrophosphate synthase GACE1337 from the hyperthermophilic archaeon Geoglobus acetivorans. Extremophiles. 2018 Nov;22(6):877-888. doi: 10.1007/s00792-018-1044-5. Epub 2018 , Jul 30. PMID:30062607 doi:http://dx.doi.org/10.1007/s00792-018-1044-5
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