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| | <StructureSection load='5jg4' size='340' side='right'caption='[[5jg4]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='5jg4' size='340' side='right'caption='[[5jg4]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JG4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JG4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fia|5fia]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpymg_00644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [https://pdbe.org/5jg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [https://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33152]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Beyrakhova, K]] | |
| - | [[Category: Cygler, M]] | |
| - | [[Category: Straaten, K van]] | |
| - | [[Category: Bacterial effector]] | |
| | [[Category: Legionella pneumophila]] | | [[Category: Legionella pneumophila]] |
| - | [[Category: Phosphate binding]] | + | [[Category: Beyrakhova K]] |
| - | [[Category: Signaling protein]] | + | [[Category: Cygler M]] |
| | + | [[Category: Van Straaten K]] |
| Structural highlights
Publication Abstract from PubMed
Legionella pneumophila is a causative agent of a severe pneumonia, known as Legionnaires' disease. Legionella's pathogenicity is mediated by specific virulence factors, called bacterial effectors, which are injected into the invaded host cell by the bacterial Type IV Secretion System. Bacterial effectors are involved in complex interactions with the components of the host cell immune and signaling pathways, that eventually lead to bacterial survival and replication inside the mammalian cell. Structural and functional studies of bacterial effectors are therefore crucial for elucidating the mechanisms of Legionella virulence. Here we describe the crystal structure of the LpiR1 (Lpg0634) effector protein and investigate the effects of its overexpression in mammalian cells. LpiR1 is an alpha-helical protein, that consists of two similar domains aligned in an antiparallel fashion. The hydrophilic cleft between the domains might serve as a binding site for a potential host cell interaction partner. LpiR1 binds phosphate group at a conserved site and is stabilized by Mn2+, Ca2+ or Mg2+ ions. When overexpressed in mammalian cells, a GFP-LpiR1 fusion protein is localized in the cytoplasm. Intracellular signaling antibody array analysis revealed small changes in the phosphorylation state of several components of the Akt signaling pathway in HEK293T cells overexpressing LpiR1.
Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila.,Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M. Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila. J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543 doi:http://dx.doi.org/10.1074/jbc.M115.708701
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