Protein Hfq

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Current revision (10:18, 14 September 2023) (edit) (undo)
 
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The biological assembly of Protein Hfq from ''E. coli'' is <scene name='87/875687/Cv/2'>homohexamer</scene> (PDB code [[4ht9]]). The 3D structure of the complex between Hfq and <scene name='87/875687/Cv/5'>poly(A) RNA and poly(U) RNA</scene> shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows <scene name='87/875687/Cv/4'>hydrogen bonding to Gln and Lys</scene>. The <scene name='87/875687/Cv/6'>R site shows stacking interactions as well as hydrogen bonding</scene>. The N site does not show any interaction. <scene name='87/875687/Cv/7'>The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions</scene><ref>PMID:23605038</ref>.
The biological assembly of Protein Hfq from ''E. coli'' is <scene name='87/875687/Cv/2'>homohexamer</scene> (PDB code [[4ht9]]). The 3D structure of the complex between Hfq and <scene name='87/875687/Cv/5'>poly(A) RNA and poly(U) RNA</scene> shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows <scene name='87/875687/Cv/4'>hydrogen bonding to Gln and Lys</scene>. The <scene name='87/875687/Cv/6'>R site shows stacking interactions as well as hydrogen bonding</scene>. The N site does not show any interaction. <scene name='87/875687/Cv/7'>The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions</scene><ref>PMID:23605038</ref>.
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</StructureSection>
 
== 3D Structures of protein Hfq ==
== 3D Structures of protein Hfq ==
[[Protein Hfq 3D structures]]
[[Protein Hfq 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 
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{{#tree:id=OrganizedByTopic|openlevels=0|
 
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*Protein Hfq
 
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**[[1hk9]], [[2y90]], [[2yht]], [[3qhs]], [[4rcb]], [[4rcc]], [[6bdg]] – EcHfq – ''Escherichia coli'' <br />
 
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**[[4jli]], [[4jri]], [[4jrk]], [[4juv]] – EcHfq (mutant)<br />
 
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**[[1u1s]], [[1u1t]], [[4j6y]], [[6xyj]] – PaHfq – ''Pseudomonas aeruginosa''<br />
 
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**[[3inz]], [[3m4g]], [[4mml]], [[4mmk]], [[5i21]] – PaHfq (mutant)<br />
 
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**[[2ylb]] – StHfq – ''Salmonella typhimurium''<br />
 
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**[[6gwk]] – Hfq – ''Caulobacter crescentus''<br />
 
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**[[4nl2]] – LmHfq – ''Listeria monocytogenes''<br />
 
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**[[4noy]] – LmHfq (mutant)<br />
 
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**[[5szd]] – AaHfq – ''Aquifex aeolicus''<br />
 
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**[[3sb2]] – Hfq - ''Herbaspirillum seropedicae''<br />
 
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**[[1kq1]] – SaHfq – ''Staphylococcus aureus''<br />
 
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**[[2qtx]], [[4x9c]] – MjHfq – ''Methanococcus jannaschii''<br />
 
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**[[3hfn]] – Hfq - ''Anabaena''<br />
 
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*Protein Hfq complex with nucleotide
 
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**[[3res]] – EcHfq + ADP<br />
 
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**[[3qo3]] – EcHfq + ATP<br />
 
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**[[4pno]] – EcHfq + UMP<br />
 
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**[[4j6x]] – PaHfq + UTP<br />
 
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**[[3qui]] – PaHfq + ADPNP<br />
 
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**[[4j5y]] – PaHfq + ATP<br />
 
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**[[4j6w]] – PaHfq + CTP<br />
 
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**[[2ylc]] – StHfq + UMP<br />
 
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**[[5dy9]] – MjHfq (mutant) + AMP<br />
 
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**[[4x9d]] – MjHfq + UMP<br />
 
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*Protein Hfq other complexes
 
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**[[6qlb]] – EcHfq + calpain <br />
 
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**[[3vu3]] – EcHfq + catalase <br />
 
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**[[3gib]], [[4ht8]] – EcHfq + poly(A)<br />
 
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**[[4ht9]], [[5new]] – EcHfq + poly(A) + poly(U)<br />
 
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**[[3rer]] – EcHfq + poly(U) + ADP<br />
 
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**[[4qvc]], [[4qvd]] – EcHfq + RNA<br />
 
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**[[5uk7]] – EcHfq + DNA<br />
 
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**[[7ogm]] – EcHfq + PNPase + RNA – Cryo EM<br />
 
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**[[3ahu]], [[3hsb]] – Hfq + RNA – ''Bacillus subtilis''<br />
 
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**[[6o1k]], [[6o1l]], [[6o1m]] – PaHfq + Crc + RNA – Cryo EM<br />
 
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**[[4v2s]] – StHfq + sRNA<br />
 
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**[[4y91]] – Hfq + poly(U) – ''Thermotoga maritima''<br />
 
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**[[4nl3]] – LmHfq + poly(U) <br />
 
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**[[5sze]] – AaHfq + poly(U) <br />
 
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**[[1kq2]] – SaHfq + RNA <br />
 
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}}
 
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of E. coli hexameric Hfq complex with poly(A) RNA and poly(U) RNA (PDB code 4ht9)

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Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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