1muh
From Proteopedia
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[[Image:1muh.gif|left|200px]] | [[Image:1muh.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1muh| PDB=1muh | SCENE= }} | |
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'''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA''' | '''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MUH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry | + | 1MUH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1f3i 1f3i]. The following page contains interesting information on the relation of 1MUH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb84_1.html Transposase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Reznikoff, W S.]] | [[Category: Reznikoff, W S.]] | ||
[[Category: Thoden, J B.]] | [[Category: Thoden, J B.]] | ||
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | [[Category: | + | [[Category: Ribonuclease h-like motif]] |
| - | [[Category: | + | [[Category: Synaptic complex]] |
| - | [[Category: | + | [[Category: Transposase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:43:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:43, 2 May 2008
CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA
Overview
Genomic evolution has been profoundly influenced by DNA transposition, a process whereby defined DNA segments move freely about the genome. Transposition is mediated by transposases, and similar events are catalyzed by retroviral integrases such as human immunodeficiency virus-1 (HIV-1) integrase. Understanding how these proteins interact with DNA is central to understanding the molecular basis of transposition. We report the three-dimensional structure of prokaryotic Tn5 transposase complexed with Tn5 transposon end DNA determined to 2.3 angstrom resolution. The molecular assembly is dimeric, where each double-stranded DNA molecule is bound by both protein subunits, orienting the transposon ends into the active sites. This structure provides a molecular framework for understanding many aspects of transposition, including the binding of transposon end DNA by one subunit and cleavage by a second, cleavage of two strands of DNA by a single active site via a hairpin intermediate, and strand transfer into target DNA.
About this Structure
1MUH is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1f3i. The following page contains interesting information on the relation of 1MUH with [Transposase]. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the Tn5 synaptic complex transposition intermediate., Davies DR, Goryshin IY, Reznikoff WS, Rayment I, Science. 2000 Jul 7;289(5476):77-85. PMID:10884228 Page seeded by OCA on Sat May 3 01:43:59 2008
