1mwg
From Proteopedia
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'''STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE''' | '''STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWG OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703939 8703939] | Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703939 8703939] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Santalucia, J.]] | [[Category: Santalucia, J.]] | ||
[[Category: Turner, D H.]] | [[Category: Turner, D H.]] | ||
[[Category: Wu, M.]] | [[Category: Wu, M.]] | ||
| - | [[Category: | + | [[Category: Duplex]] |
| - | [[Category: | + | [[Category: G:a mismatch]] |
| - | [[Category: | + | [[Category: Ribonucleic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:48, 2 May 2008
STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939 Page seeded by OCA on Sat May 3 01:48:07 2008
