1mwj

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[[Image:1mwj.gif|left|200px]]
[[Image:1mwj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1mwj |SIZE=350|CAPTION= <scene name='initialview01'>1mwj</scene>, resolution 2.85&Aring;
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The line below this paragraph, containing "STRUCTURE_1mwj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=DU:2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>DU</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= MUG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=
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{{STRUCTURE_1mwj| PDB=1mwj | SCENE= }}
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|RELATEDENTRY=[[1mug|1MUG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwj OCA], [http://www.ebi.ac.uk/pdbsum/1mwj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mwj RCSB]</span>
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}}
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'''Crystal Structure of a MUG-DNA pseudo substrate complex'''
'''Crystal Structure of a MUG-DNA pseudo substrate complex'''
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[[Category: Scharer, O.]]
[[Category: Scharer, O.]]
[[Category: Verdine, G L.]]
[[Category: Verdine, G L.]]
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[[Category: non-hydrolysable dna-complex]]
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[[Category: Non-hydrolysable dna-complex]]
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[[Category: rossmann fold]]
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[[Category: Rossmann fold]]
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[[Category: uracil recognition.]]
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[[Category: Uracil recognition.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:22:06 2008''
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Revision as of 22:48, 2 May 2008

Image:1mwj.gif

Template:STRUCTURE 1mwj

Crystal Structure of a MUG-DNA pseudo substrate complex


Overview

The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family of DNA glycosylases that initiate base-excision repair of G:U/T mismatches. Despite low sequence homology, the MUG/TDG enzymes are structurally related to the uracil-DNA glycosylase enzymes, but have a very different mechanism for substrate recognition. We have now determined the crystal structure of the Escherichia coli MUG enzyme complexed with an oligonucleotide containing a non-hydrolysable deoxyuridine analogue mismatched with guanine, providing the first structure of an intact substrate-nucleotide productively bound to a hydrolytic DNA glycosylase. The structure of this complex explains the preference for G:U over G:T mispairs, and reveals an essentially non-specific pyrimidine-binding pocket that allows MUG/TDG enzymes to excise the alkylated base, 3, N(4)-ethenocytosine. Together with structures for the free enzyme and for an abasic-DNA product complex, the MUG-substrate analogue complex reveals the conformational changes accompanying the catalytic cycle of substrate binding, base excision and product release.

About this Structure

1MWJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a thwarted mismatch glycosylase DNA repair complex., Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH, EMBO J. 1999 Dec 1;18(23):6599-609. PMID:10581234 Page seeded by OCA on Sat May 3 01:48:21 2008

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