1mx0
From Proteopedia
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[[Image:1mx0.gif|left|200px]] | [[Image:1mx0.gif|left|200px]] | ||
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'''Structure of topoisomerase subunit''' | '''Structure of topoisomerase subunit''' | ||
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==Reference== | ==Reference== | ||
Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12505993 12505993] | Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12505993 12505993] | ||
| - | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus shibatae]] | [[Category: Sulfolobus shibatae]] | ||
[[Category: Berger, J M.]] | [[Category: Berger, J M.]] | ||
[[Category: Corbett, K D.]] | [[Category: Corbett, K D.]] | ||
| - | [[Category: | + | [[Category: Ghkl atpase]] |
| - | [[Category: | + | [[Category: Topoisomerase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:49:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:49, 2 May 2008
Structure of topoisomerase subunit
Overview
Type IIA and type IIB topoisomerases each possess the ability to pass one DNA duplex through another in an ATP-dependent manner. The role of ATP in the strand passage reaction is poorly understood, particularly for the type IIB (topoisomerase VI) family. We have solved the structure of the ATP-binding subunit of topoisomerase VI (topoVI-B) in two states: an unliganded monomer and a nucleotide-bound dimer. We find that topoVI-B is highly structurally homologous to the entire 40-43 kDa ATPase region of type IIA topoisomerases and MutL proteins. Nucleotide binding to topoVI-B leads to dimerization of the protein and causes dramatic conformational changes within each protomer. Our data demonstrate that type IIA and type IIB topoisomerases have descended from a common ancestor and reveal how ATP turnover generates structural signals in the reactions of both type II topoisomerase families. When combined with the structure of the A subunit to create a picture of the intact topoisomerase VI holoenzyme, the ATP-driven motions of topoVI-B reveal a simple mechanism for strand passage by the type IIB topoisomerases.
About this Structure
1MX0 is a Single protein structure of sequence from Sulfolobus shibatae. Full crystallographic information is available from OCA.
Reference
Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:12505993 Page seeded by OCA on Sat May 3 01:49:17 2008
