1myw
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1myw.gif|left|200px]] | [[Image:1myw.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1myw", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1myw| PDB=1myw | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY''' | '''CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY''' | ||
| Line 30: | Line 27: | ||
[[Category: Nagai, T.]] | [[Category: Nagai, T.]] | ||
[[Category: Rekas, A.]] | [[Category: Rekas, A.]] | ||
| - | [[Category: | + | [[Category: Beta-barrel]] |
| - | [[Category: | + | [[Category: Bioluminescence]] |
| - | [[Category: | + | [[Category: Chromophore]] |
| - | [[Category: | + | [[Category: Green fluorescent protein]] |
| - | [[Category: | + | [[Category: Improved maturation]] |
| - | [[Category: | + | [[Category: Photoactive protein]] |
| - | [[Category: | + | [[Category: Yellow-emission variant]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:52:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:52, 2 May 2008
CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY
Overview
Yellow emission variants of green fluorescent protein (GFP) have been found useful in a variety of applications in biological systems due to their red-shifted emission spectrum and sensitivity to environmental parameters, such as pH and ionic strength. However, slow maturation properties and new requirements for more intense fluorescence necessitated further mutagenesis studies of these proteins. Venus, a new variant with improved maturation and brightness, as well as reduced environmental dependence, was recently developed by introducing five mutations into the well characterized variant, enhanced yellow fluorescent protein (EYFP). In this paper, we present the crystal structure of Venus at 2.2 A resolution, which enabled us to correlate its novel features with these mutation points. The rearrangement of several side chains near the chromophore, initiated by the F46L mutation, was found to improve maturation at 37 degrees C by removing steric and energetic constraints, which may hinder folding of the polypeptide chain, and by accelerating the oxidation of the Calpha-Cbeta bond of Tyr(66) during chromophore formation. M153T, V163A, and S175G were also found to improve the rate of maturation by creating regions of greater flexibility. F64L induced large conformational changes in the molecule, leading to the removal of halide sensitivity by preventing ion access to the binding site.
About this Structure
1MYW is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.
Reference
Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity., Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M, J Biol Chem. 2002 Dec 27;277(52):50573-8. Epub 2002 Oct 4. PMID:12370172 Page seeded by OCA on Sat May 3 01:52:52 2008
